caa a22 4500 469027703 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00233360 doi (NATIONALLICENCE)springer-10.1007/BF00233360 Transformation of rapid cycling cabbage ( Brassica oleracea var. capitata) with Agrobacterium rhizogenes [Elektronische Daten] [Pierre Berthomieu, Lise Jouanin] Summary: Genetically transformed cabbage (Brassica oleracea var. capitata) roots were obtained after inoculation with two engineered Agrobacterium rhizogenes strains, each harbouring a plant selectable marker gene in their T-DNA. Axenic root clones resistant to kanamycin or hygromycin B were established, most of which did not exhibit the phenotypic characteristics of Ri-transformed roots. Shoot regeneration was induced from roots after treatment with 2,4-dichlorophenoxyacetic acid (2,4-D). The resulting plants exhibited various phenotypes: some looked normal, while others showed the transformed phenotype observed in other species. Direct evidence for genetic transformation was obtained by molecular hybridization. The trait was transmitted to the progeny. Transformed cabbage plants can be obtained within 6 months using this approach. Springer-Verlag, 1992 Brassica oleracea nationallicence rapid cycling cabbage nationallicence transformation nationallicence Agrobacterium rhizogenes nationallicence Berthomieu Pierre Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198, Gif sur Yvette Cédex, France aut Jouanin Lise Laboratoire de Biologie Cellulaire, INRA, Route de Saint-Cyr, 78026, Versailles Cédex, France aut Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 334-338 0721-7714 11:7<334 1992 11 299 https://doi.org/10.1007/BF00233360 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233360 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berthomieu Pierre Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198, Gif sur Yvette Cédex, France aut NATIONALLICENCE 700 1- Jouanin Lise Laboratoire de Biologie Cellulaire, INRA, Route de Saint-Cyr, 78026, Versailles Cédex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 334-338 0721-7714 11:7<334 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer