caa a22 4500 469027738 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00233367 doi (NATIONALLICENCE)springer-10.1007/BF00233367 Effects of agrobacterial oncogenes in kidney vetch ( Anthyllis vulneraria L.) [Elektronische Daten] [Jiří Stiller, Vít sinec, Stanislav Svoboda, Blanka Němcová, Ivana Macháčková] Kidney vetch seedlings were induced to form hairy roots by inoculating their mesocotyls with the wild-type strain 15834 of Agrobacterium rhizogenes or with the A. tumefaciens strain C58C1 containing a binary vector system (the pRiA4b as a helper and the vector pCB1346 bearing a pTiC58-derived isopentenyl transferase gene (ipt, cytokinin biosynthetic gene) under control of its native regulatory sequences). Transgenic lines of three distinct phenotypes were selected: (i) Typically, the pRi15834-transformed tissues were stabilized in vitro and maintained for long periods as aseptic, fast-growing, hormone-independent, plagiotropic hairy root cultures which never regenerated shoots and lost the ability to synthesize opines. Their genomic DNA contained both the TL- and the TR-DNA. (ii) One of the HR-lines transgenic for the T-DNA of pRi15834 (named 52AV34) started to regenerate spontaneously into teratomous shoots. The shoots were found to produce opines and both the TL and TR parts of T-DNA were found to be partly deleted and/or rearranged. They contained phytohormones in similar levels as those found in seed-born shoots. (iii) A practically identical morphogenic response as in the line 52AV34 was observed in the clone 27AV46. However, its shooty, dark-green, slow-growing teratomas were proven to be kanamycin-resistant, opine-producing, and double-transformed by the pRiA4b sequences and the ipt gene. They over-produced auxins as well as cytokinins (mainly indoleacetylaspartic acid and ribosides of zeatin and isopentenyladenine). Springer-Verlag, 1992 Stiller Jiří Department of Nitrogen Fixation, Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czechoslovakia aut sinec Vít Department of Nitrogen Fixation, Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czechoslovakia aut Svoboda Stanislav Department of Nitrogen Fixation, Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czechoslovakia aut Němcová Blanka Department of Nitrogen Fixation, Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czechoslovakia aut Macháčková Ivana Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ke dvoru 15, 160 00, Prague 6, Czechoslovakia aut Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 363-367 0721-7714 11:7<363 1992 11 299 https://doi.org/10.1007/BF00233367 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233367 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stiller Jiří Department of Nitrogen Fixation, Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czechoslovakia aut NATIONALLICENCE 700 1- sinec Vít Department of Nitrogen Fixation, Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czechoslovakia aut NATIONALLICENCE 700 1- Svoboda Stanislav Department of Nitrogen Fixation, Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czechoslovakia aut NATIONALLICENCE 700 1- Němcová Blanka Department of Nitrogen Fixation, Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Branišovská 31, 37005, České Budějovice, Czechoslovakia aut NATIONALLICENCE 700 1- Macháčková Ivana Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ke dvoru 15, 160 00, Prague 6, Czechoslovakia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 363-367 0721-7714 11:7<363 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer