caa a22 4500 469027746 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00233366 doi (NATIONALLICENCE)springer-10.1007/BF00233366 Shoot regeneration in stem expiants and its amenability to Agrobacterium tumefaciens mediated gene transfer in Brassica carinata [Elektronische Daten] [S. Narasimhulu, P. Kirti, T. Mohapatra, Shyam Prakash, V. Chopra] Immature stem segments of seven different genotypes of Brassica carinata produced shoots with variable frequencies when cultured in MS medium with BAP and picloram at 0.2 mg/l each. Line 171, which produced shoots with 100% efficiency from both cut ends of the expiant, was selected for testing the amenability of this regeneration protocol for genetic transformation. A non-oncogenic Agrobacterium tumefaciens containing plasmid PCV 730, a binary vector carrying resistance genes for kanamycin and hygromycin, was used. A cocultivation period of 4 d with a bacterial concentration of approximately 2.5×10 cells/ml, followed by a recovery period of 2 d, produced transformed shoots that could be selected and rooted in the presence of kanamycin at 15 mg/l. Transformation was confirmed by neomycin phospho-transferase assay and Southern blot analysis. Seed analysis of transformed plants indicated that kanamycin resistance was inherited in the progeny. Springer-Verlag, 1992 Brassica carinata nationallicence Agrobacterium tumefaciens nationallicence Shoot regeneration nationallicence Gene transfer nationallicence BAP : 6-Benzylaminopurine nationallicence Kn : Kinetin nationallicence IAA : Indole-3-acetic acid nationallicence IBA : Indole-3-butyric acid nationallicence NAA : ∝-Naphthaleneacetic acid nationallicence ATP : Adenosine triphosphate nationallicence MS : Murashige and Skoog (1962) medium nationallicence Narasimhulu S. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Kirti P. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Mohapatra T. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Prakash Shyam Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Chopra V. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 359-362 0721-7714 11:7<359 1992 11 299 https://doi.org/10.1007/BF00233366 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233366 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Narasimhulu S. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Kirti P. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Mohapatra T. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Prakash Shyam Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Chopra V. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 359-362 0721-7714 11:7<359 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer