caa a22 4500 469027754 CHVBK 20180323132734.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00233359 doi (NATIONALLICENCE)springer-10.1007/BF00233359 Plant regeneration from sugarbeet ( Beta vulgaris L.) hypocotyls cultured in vitro and flow cytometric nuclear DNA analysis of regenerants [Elektronische Daten] [Benoit Jacq, Thierry Tétu, Rajbir Sangwan, Ad De Laat, Brigitte Sangwan-Norreel] A simple and reproducible protocol for regeneration of sugarbeet plants from hypocotyl expiants derived from 21 day-old-seedlings has been developed. Expiants were cultured on MS medium containing 0.3 mg/l N6-Benzylaminopurine, 0.1 mg/l Naphthalene Acetic Acid, 50 mg/l adenine and 0.5% (w/v) fructose, 0.5% (w/v) sucrose and 0.5% (w/v) glucose to induce the formation of organogenic calli (2.3% to 46.5% organogenic efficiency, depending on populations). Shoot formation was induced in callus cultures of more than 1600 genotypes. Physiological age affected culture response and different genotypes had different temperature optima for organogenesis. Following transfer of regenerated plants to the greenhouse, DNA determinations were made to study the stability of ploidy. Differences in ploidy were observed in plants derived from both shortterm and long-term callus cultures; diploid true-to-type regenerants were 96% and 83%, respectively, from shortterm and long-term callus cultures. Springer-Verlag, 1992 Jacq Benoit Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France aut Tétu Thierry Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France aut Sangwan Rajbir Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France aut De Laat Ad D J Van Der Have B. V., Plant Breeding Station, Rilland, Netherlands aut Sangwan-Norreel Brigitte Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France aut Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 329-333 0721-7714 11:7<329 1992 11 299 https://doi.org/10.1007/BF00233359 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233359 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jacq Benoit Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France aut NATIONALLICENCE 700 1- Tétu Thierry Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France aut NATIONALLICENCE 700 1- Sangwan Rajbir Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France aut NATIONALLICENCE 700 1- De Laat Ad D J Van Der Have B. V., Plant Breeding Station, Rilland, Netherlands aut NATIONALLICENCE 700 1- Sangwan-Norreel Brigitte Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 329-333 0721-7714 11:7<329 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer