caa a22 4500 469027789 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00233358 doi (NATIONALLICENCE)springer-10.1007/BF00233358 Development of the particle inflow gun for DNA delivery to plant cells [Elektronische Daten] [John Finer, Philippe Vain, Mark Jones, Michael McMullen] Summary: A simple and inexpensive particle bombardment device was constructed for delivery of DNA to plant cells. The Particle Inflow Gun (PIG) is based on acceleration of DNA-coated tungsten particles using pressurized helium in combination with a partial vacuum. The particles are accelerated directly in a helium stream rather than being supported by a macrocarrier. Bombardment parameters were partially optimized using transient expression assays of a ß-glucuronidase gene in maize embryogenic suspension culture and cowpea leaf tissues. High levels of transient expression of the ß-glucuronidase gene were obtained following bombardment of embryogenic suspension cultures of corn and soybean, and leaf tissue of cowpea. Stable transformation of embryogenic tissue of soybean has also been obtained using this bombardment apparatus. Springer-Verlag, 1992 Finer John Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA aut Vain Philippe Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA aut Jones Mark Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA aut McMullen Michael Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA aut Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 323-328 0721-7714 11:7<323 1992 11 299 https://doi.org/10.1007/BF00233358 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233358 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Finer John Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA aut NATIONALLICENCE 700 1- Vain Philippe Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA aut NATIONALLICENCE 700 1- Jones Mark Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA aut NATIONALLICENCE 700 1- McMullen Michael Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 323-328 0721-7714 11:7<323 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer