caa a22 4500 469027800 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00233365 doi (NATIONALLICENCE)springer-10.1007/BF00233365 Matrix-associated DNA from maize is enriched in repetitive sequences [Elektronische Daten] [Lubomir Stoilov, Valeria Mirkova, Jordanka Zlatanova, Lalio Djondjurov] In order to elucidate some features of the topological organization of DNA within the plant nucleus, DNA fragments involved in the attachment of the DNA loops to the nuclear matrix in maize were studied. The matrix-associated DNA from dry embryo and meristematic cells after extensive digestion with DNase I and high salt treatment was about 2% of the total DNA, sized within the range of 50 and 250 bp. This DNA was found to be enriched in repetitive DNA sequences, both for nuclei from dry embryo and meristematic cells. The loop size of the DNA in cells of Zea mays appeared to be between 5 and 25 kbp. Springer-Verlag, 1992 Zea mays nationallicence Matrix-associated nationallicence DNA nationallicence repetitive sequences nationallicence DNA loops nationallicence EDTA : Diamino-ethanetetraacetic acid nationallicence EtBr : Ethidium bromide nationallicence LIS : Lithium diiodosalicylate nationallicence PMSF : Phenylmethylsulfonyl fluoride nationallicence SDS : Sodium dodecyl sulfate nationallicence Stoilov Lubomir Institute of Genetics, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria aut Mirkova Valeria Institute of Genetics, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria aut Zlatanova Jordanka Institute of Genetics, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria aut Djondjurov Lalio Institute of Molecular Biology, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria aut Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 355-358 0721-7714 11:7<355 1992 11 299 https://doi.org/10.1007/BF00233365 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233365 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stoilov Lubomir Institute of Genetics, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Mirkova Valeria Institute of Genetics, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Zlatanova Jordanka Institute of Genetics, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Djondjurov Lalio Institute of Molecular Biology, Bulgarian Academy of Sciences, PO Box 96, 1113, Sofia, Bulgaria aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/7(1992-07-01), 355-358 0721-7714 11:7<355 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer