caa a22 4500 469027819 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00236387 doi (NATIONALLICENCE)springer-10.1007/BF00236387 A plant selectable marker gene based on the detoxification of the herbicide dalapon [Elektronische Daten] [Vicky Buchanan-Wollaston, Andrea Snape, Frank Cannon] Summary: A gene from Pseudomonas putida coding for a dehalogenase capable of degrading 2,2 dichloropropionic acid (2,2DCPA), the active ingredient of the herbicide dalapon, has been isolated and characterised. In plant transformation experiments the gene was shown to confer resistance to 2,2DCPA at a tissue culture level where 2,2DCPA could be used to select for transformants. At the whole plant level, transformed plants showed resistance to 2,2DCPA at concentrations up to 5 times the recommended dose rate of dalapon when it was sprayed on their leaves. At lower concentrations, the herbicide caused a non-lethal yellowing of sensitive plants which clearly distinguished them from resistant plants. The mode of action of chlorinated aliphatic acids is not known but they probably affect many enzyme pathways. The results described here are the first example of engineering a plant resistant to a herbicide that does not have one specific enzyme as its target site. This gene has several advantages as a marker in plant breeding and genetic studies. For example, the herbicide is readily available and has low toxicity, transformants can be selected at both the tissue culture and the whole plant level, a large number of transformed plants can easily be screened even in the field, and there is a very low probability of selecting spontaneous mutants. Springer-Verlag, 1992 Buchanan-Wollaston Vicky BioTechnica International Inc., 85, Bolton Street, 02140, Cambridge, MA, USA aut Snape Andrea BioTechnica International Inc., 85, Bolton Street, 02140, Cambridge, MA, USA aut Cannon Frank BioTechnica International Inc., 85, Bolton Street, 02140, Cambridge, MA, USA aut Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 627-631 0721-7714 11:12<627 1992 11 299 https://doi.org/10.1007/BF00236387 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236387 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buchanan-Wollaston Vicky BioTechnica International Inc., 85, Bolton Street, 02140, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Snape Andrea BioTechnica International Inc., 85, Bolton Street, 02140, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Cannon Frank BioTechnica International Inc., 85, Bolton Street, 02140, Cambridge, MA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 627-631 0721-7714 11:12<627 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer