caa a22 4500 469027827 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00236385 doi (NATIONALLICENCE)springer-10.1007/BF00236385 Plant regeneration from haploid cell suspension-derived protoplasts of Mediterranean rice ( Oryza sativa L. cv. Miara) [Elektronische Daten] [E. Guiderdoni, H. Chaïr] More than 750 plants were regenerated from protoplasts isolated from microspore callus-derived cell suspensions of the Mediterranean japonica rice Miara, using a nurse-feeder technique and N6-based culture medium. The mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. Flow cytometric evaluation of the DNA contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. Contrastingly, the DNA contents of leaf cell nuclei of the regenerated protoclones ranged from 1C to 5C including 60% 2C plants. This was consistent with the morphological type and the fertility of the mature plants. These results and the absence of chimeric plants suggest that polyploidization occurred during the early phase of protoplast culture. Springer-Verlag, 1992 Mediterranean Rice nationallicence Oryza sativa L nationallicence haploid protoplasts nationallicence regeneration nationallicence protoclonal variation nationallicence BAP : 6-benzylamino purine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence FDA : fluorescein diacetate nationallicence GA3 : gibberellic acid nationallicence NAA : α-naphthaleneacetic acid nationallicence PAS : periodic acid Schiff nationallicence PCM : protoplast culture medium nationallicence PCV : packed cell volume nationallicence Guiderdoni E. IRAT-CIRAD, BIOTROP-GERDAT Laboratory, BP5035, 34032, Montpellier Cédex 01, France aut Chaïr H. IRAT-CIRAD, BIOTROP-GERDAT Laboratory, BP5035, 34032, Montpellier Cédex 01, France aut Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 618-622 0721-7714 11:12<618 1992 11 299 https://doi.org/10.1007/BF00236385 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236385 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guiderdoni E. IRAT-CIRAD, BIOTROP-GERDAT Laboratory, BP5035, 34032, Montpellier Cédex 01, France aut NATIONALLICENCE 700 1- Chaïr H. IRAT-CIRAD, BIOTROP-GERDAT Laboratory, BP5035, 34032, Montpellier Cédex 01, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 618-622 0721-7714 11:12<618 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer