caa a22 4500 469027835 CHVBK 20190126092112.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00236380 doi (NATIONALLICENCE)springer-10.1007/BF00236380 Characteristics of growth and tropane alkaloid production in Hyoscyamus albus hairy roots transformed with Agrobacterium rhizogenes A4 [Elektronische Daten] [Philippe Christen, Toshio Aoki, Koichiro Shimomura] Summary: Hairy root culture of Hyoscyamus albus was established by transformation with Agrobacterium rhizogenes strain A4. The growth and production of five tropane alkaloids were investigated under various culture conditions. Among the four basal culture media tested, Woody Plant medium was the best for growth of the hairy roots, but a high amount of tropane alkaloids was obtained with Gamborg's B5 medium. Sucrose concentration in B5 medium had little effect on the growth, while 3% sucrose was suitable for the alkaloid production. Addition of KNO3 to Woody Plant medium affected the growth, whereas the alkaloid content was not markedly improved. Supplement of some metal ions to B5 medium stimulated the alkaloid production. In particular, Cu2+ remarkably enhanced both the growth and the alkaloid yield. The hairy roots cultured under 16 h/day light survived for more than 32 days compared with those cultured in the dark. Springer-Verlag, 1992 Hyoscyamus albus nationallicence Agrobacterium rhizogenes nationallicence Hairy root culture nationallicence Tropane alkaloids nationallicence EDTA : ethylenediaminetetraacetic acid nationallicence HPLC : high performance liquid chromatography nationallicence MeOH : methanol nationallicence MS medium : Murashige and Skoog medium nationallicence WP medium : McCown's Woody Plant medium nationallicence B5 medium : Gamborg B5 medium nationallicence wt : weight nationallicence Christen Philippe Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut Aoki Toshio Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut Shimomura Koichiro Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 597-600 0721-7714 11:12<597 1992 11 299 https://doi.org/10.1007/BF00236380 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236380 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Christen Philippe Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Aoki Toshio Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Shimomura Koichiro Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 597-600 0721-7714 11:12<597 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer