caa a22 4500 469027843 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00236381 doi (NATIONALLICENCE)springer-10.1007/BF00236381 Transgenic turf-type tall fescue ( Festuca amndinacea Schreb.) plants regenerated from protoplasts [Elektronische Daten] [Sam-Bong Ha, Fang-Sheng Wu, Tracy Thorne] To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture protoplasts and plants were regenerated from protoplasts of tall fescue cultured cells. To develop transgenic tall fescue plants, the hygromycin resistance gene and the β-glucuronidase gene were introduced into the tall fescue protoplasts by electroporation. A high concentration (200 mg/l) of hygromycin was required to select transformed cells because of the high level of endogenous resistance to the antibiotic in tall fescue. Most of the transformed cells exhibited GUS activity and several plants were regenerated from these cells. The presence of introduced genes was confirmed by Southern blot hybridization of PCR amplified DNA from transgenic plants. Springer-Verlag, 1992 Ha Sam-Bong Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA aut Wu Fang-Sheng Department of Biology, Virginia Commonwealth University, 23284, Richmond, VA, USA aut Thorne Tracy Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA aut Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 601-604 0721-7714 11:12<601 1992 11 299 https://doi.org/10.1007/BF00236381 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236381 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ha Sam-Bong Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Wu Fang-Sheng Department of Biology, Virginia Commonwealth University, 23284, Richmond, VA, USA aut NATIONALLICENCE 700 1- Thorne Tracy Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 601-604 0721-7714 11:12<601 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer