caa a22 4500 469027851 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00236388 doi (NATIONALLICENCE)springer-10.1007/BF00236388 Protoplasts from cotyledon and hypocotyl of sunflower ( Helianthus annuus L.): shoot regeneration and seed production [Elektronische Daten] [Christiane Fischer, Paul Klethi, Günther Hahne] Summary: Cotyledon and hypocotyl protoplasts of Helianthus annuus inbred line 47 302 bcd were embedded in alginate and plated on L4 medium (Lenée and Chupeau 1986). After one month, the calli were transferred on MSSH regeneration medium (Murashige and Skoog 1962; Schenk and Hildebrandt 1972) where they regenerated shoots (overall efficiency 10−2%). The shoots were elongated on B5 (Gamborg et al. 1968) medium first without hormones, then supplemented with GA3 and BAP (both 0.05 mg/l). In order to overcome the difficulty to induce rooting by classical methods, the elongated shoots were grafted on a sunflower rootstock. The grafted shoots produced flowers and seeds. Different factors have been shown to have an important influence on the capacity to regenerate shoots: the genotype, the physical culture conditions at the callus regeneration step (e.g. protoplasts embedded in alginate), and the media composition. Springer-Verlag, 1992 Helianthus annuus nationallicence Protoplasts nationallicence Plant regeneration nationallicence BAP : 6-benzylaminopurine nationallicence GA3 : gibberellic acid nationallicence IBA : indole-3-butanoic acid nationallicence IAA : indole acetic acid nationallicence MES : 2-N-morpholinoethane sulfonic acid nationallicence NAA : 1-naphthalene acetic acid nationallicence 2,4D : 2,4 dichlorophenoxyacetic acid nationallicence Fischer Christiane Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg-Cedex, France aut Klethi Paul Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg-Cedex, France aut Hahne Günther Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg-Cedex, France aut Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 632-636 0721-7714 11:12<632 1992 11 299 https://doi.org/10.1007/BF00236388 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236388 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fischer Christiane Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg-Cedex, France aut NATIONALLICENCE 700 1- Klethi Paul Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg-Cedex, France aut NATIONALLICENCE 700 1- Hahne Günther Institut de Biologie Moléculaire des Plantes, CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg-Cedex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 632-636 0721-7714 11:12<632 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer