caa a22 4500 46902786X CHVBK 20180323132735.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00236384 doi (NATIONALLICENCE)springer-10.1007/BF00236384 Plant regeneration from stem cortex expiant and protoplast cultures of Brassica juncea (mustard) [Elektronische Daten] [A.-C. Bonfils, A. Sproule, J. Webb, W. Keller] Summary: Plant regeneration from stem cortex explants of 13 genotypes of Brassica juncea was assessed. Regeneration was strongly affected by genotype, as up to 50.6 shoots were produced per 100 calli of the most responsive line (Blaze), whereas no shoots were obtained from less responsive lines (Zeml, Vniimk351). Blaze was chosen for B. juncea stem cortex protoplast isolation. After one week of culture, 11-14% of the cells had divided, and about 0.002% produced 1-2 mm colonies within 6 weeks. Up to 7% of these colonies gave rise to shoots upon transfer to plant regeneration medium. Springer-Verlag, 1992 Brassica juncea nationallicence Expiants nationallicence Protoplasts nationallicence Regeneration nationallicence 2,4-D : 2,4-dichlorophenoxyaceticacid nationallicence BAP : 6-benzylaminopurine nationallicence FDA : fluorescein diacetate nationallicence IAA : indole acetic acid nationallicence IBA : indole butyric acid nationallicence 2ip : 2-isopentenyl-adenine nationallicence NAA : 1-naphthaleneacetic acid nationallicence TD : thidiazuron nationallicence Bonfils A.-C Biology Department, Tory Bldg., Carleton University, K1S 5B6, Ottawa, Ont., Canada aut Sproule A. Biology Department, Tory Bldg., Carleton University, K1S 5B6, Ottawa, Ont., Canada aut Webb J. Biology Department, Tory Bldg., Carleton University, K1S 5B6, Ottawa, Ont., Canada aut Keller W. Plant Research Centre, Bldg. 21, Agriculture Canada, K1A OC6, Ottawa, Ont., Canada aut Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 614-617 0721-7714 11:12<614 1992 11 299 https://doi.org/10.1007/BF00236384 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236384 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bonfils A.-C Biology Department, Tory Bldg., Carleton University, K1S 5B6, Ottawa, Ont., Canada aut NATIONALLICENCE 700 1- Sproule A. Biology Department, Tory Bldg., Carleton University, K1S 5B6, Ottawa, Ont., Canada aut NATIONALLICENCE 700 1- Webb J. Biology Department, Tory Bldg., Carleton University, K1S 5B6, Ottawa, Ont., Canada aut NATIONALLICENCE 700 1- Keller W. Plant Research Centre, Bldg. 21, Agriculture Canada, K1A OC6, Ottawa, Ont., Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 614-617 0721-7714 11:12<614 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer