caa a22 4500 469027878 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00236382 doi (NATIONALLICENCE)springer-10.1007/BF00236382 Bioreactor studies on the effect of dissolved oxygen concentrations on growth and differentiation of carrot ( Daucus carota L.) cell cultures [Elektronische Daten] [Véronique Jay, Simone Genestier, Jean-Claude Courduroux] A bioreactor control system was used to investigate the effects of two dissolved oxygen concentrations (10% and 100%) on the growth and differentiation of Daucus carota L. cell cultures. The strategy used allowed the dissolved oxygen concentration to be controlled without the need for changing either the agitator speed or the total gas flow rate. During the proliferation phase, reducing oxygen resulted in a lower growth rate and in a delay in sugar uptake kinetics. Nonetheless, varying levels of oxygen were observed to have no effect on the final dry biomass. The higher alcohol dehydrogenase activity obtained under reduced oxygen conditions suggests that proliferating cultures adapted to the hypoxic environment by inducing alcoholic fermentation. Cell differentiation was highly sensitive to reduced oxygen since under this condition, the somatic embryo production was inhibited by about 75%. Sugar uptake and embryo formation were also delayed. Springer-Verlag, 1992 Daucus carota nationallicence Bioreactor nationallicence Dissolved oxygen nationallicence Somatic embryogenesis nationallicence Growth and differentiation nationallicence ADH : alcohol dehydrogenase nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence DO2 : dissolved oxygen nationallicence SE : somatic embryos nationallicence Tris : tris(hydroxymethyl)-aminoethane nationallicence Jay Véronique Laboratoire de Biologie et Physiologie Végétales, Université Blaise Pascal, 4 rue Ledru, F-63038, Clermont-Ferrand Cedex 1, France aut Genestier Simone Laboratoire de Biologie et Physiologie Végétales, Université Blaise Pascal, 4 rue Ledru, F-63038, Clermont-Ferrand Cedex 1, France aut Courduroux Jean-Claude Laboratoire de Biologie et Physiologie Végétales, Université Blaise Pascal, 4 rue Ledru, F-63038, Clermont-Ferrand Cedex 1, France aut Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 605-608 0721-7714 11:12<605 1992 11 299 https://doi.org/10.1007/BF00236382 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236382 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jay Véronique Laboratoire de Biologie et Physiologie Végétales, Université Blaise Pascal, 4 rue Ledru, F-63038, Clermont-Ferrand Cedex 1, France aut NATIONALLICENCE 700 1- Genestier Simone Laboratoire de Biologie et Physiologie Végétales, Université Blaise Pascal, 4 rue Ledru, F-63038, Clermont-Ferrand Cedex 1, France aut NATIONALLICENCE 700 1- Courduroux Jean-Claude Laboratoire de Biologie et Physiologie Végétales, Université Blaise Pascal, 4 rue Ledru, F-63038, Clermont-Ferrand Cedex 1, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 605-608 0721-7714 11:12<605 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer