caa a22 4500 469027908 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00236383 doi (NATIONALLICENCE)springer-10.1007/BF00236383 Somatic embryogenesis and plantlet regeneration from cell suspension cultures of Fagus sylvatica L [Elektronische Daten] [F. Vieitez, Antonio Ballester, Ana Vieitez] Summary: Embryogenic cell suspension cultures and somatic embryos of five genotypes of beech, were obtained from aged cultures derived from immature zygotic embryos cultured on solid medium containing both 2, 4-dichlorophenoxyacetic acid and N6-benzyladenine. The origin of somatic embryos was traced from single cells. Embryos remained arrested at the globular stage on liquid media, further development was achieved after plating embryogenic aggregates on Murashige and Skoog's medium with half strength major salts supplemented with glutamine and low levels of growth regulators. Cultures of different genotypes showed significant differences in maturation frequency which was not affected by the hormone treatments assayed. The frequency of conversion of embryos into plantlets was low. This frequency increased after cold storage of embryos for up to 7 months. Springer-Verlag, 1992 Beech nationallicence Fagus sylvatica nationallicence Cell suspension nationallicence Somatic Embryogenesis nationallicence Plant regeneration nationallicence BA : N6-benzyladenine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence EtOH : ethanol nationallicence GA3 : giberrellic acid nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence MS : Murashige and Skoog (1962) nationallicence NAA : naphthalene acetic acid nationallicence WPM : woody plant medium (Lloyd and McCown, 1980) nationallicence Z : zeatin nationallicence Vieitez F. Institute de Investigaciones Agrobiológicas de Galicia. CSIC., Apartado 122, 15080, Santiago de Compostela, Spain aut Ballester Antonio Institute de Investigaciones Agrobiológicas de Galicia. CSIC., Apartado 122, 15080, Santiago de Compostela, Spain aut Vieitez Ana Institute de Investigaciones Agrobiológicas de Galicia. CSIC., Apartado 122, 15080, Santiago de Compostela, Spain aut Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 609-613 0721-7714 11:12<609 1992 11 299 https://doi.org/10.1007/BF00236383 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236383 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vieitez F. Institute de Investigaciones Agrobiológicas de Galicia. CSIC., Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Ballester Antonio Institute de Investigaciones Agrobiológicas de Galicia. CSIC., Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Vieitez Ana Institute de Investigaciones Agrobiológicas de Galicia. CSIC., Apartado 122, 15080, Santiago de Compostela, Spain aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/12(1992-11-01), 609-613 0721-7714 11:12<609 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer