caa a22 4500 469027916 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232170 doi (NATIONALLICENCE)springer-10.1007/BF00232170 Direct somatic embryogenesis and plant regeneration from protoplasts of Bupleurum scorzonerifolium Willd [Elektronische Daten] [Xia Guang-min, Li Zhongyi, Guo Guang-qin, Chen Hui-min] Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to direct embryo formation were investigated, and the nature of the callus used for protoplast preparation was found to be a critical factor. The osmotic concentration and the composition of the culture medium including the phytohormone combinations were also important. Springer-Verlag, 1992 Bupleurum scorzonerifolium nationallicence somatic embryogenesis nationallicence plant regeneration nationallicence protoplast nationallicence Guang-min Xia Department of Biology, Shandong University, 250100, Jinan, Shandong, P.R. China aut Zhongyi Li Department of Biology, Shandong University, 250100, Jinan, Shandong, P.R. China aut Guang-qin Guo Department of Biology, Shandong University, 250100, Jinan, Shandong, P.R. China aut Hui-min Chen Department of Biology, Shandong University, 250100, Jinan, Shandong, P.R. China aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 155-158 0721-7714 11:3<155 1992 11 299 https://doi.org/10.1007/BF00232170 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232170 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guang-min Xia Department of Biology, Shandong University, 250100, Jinan, Shandong, P.R. China aut NATIONALLICENCE 700 1- Zhongyi Li Department of Biology, Shandong University, 250100, Jinan, Shandong, P.R. China aut NATIONALLICENCE 700 1- Guang-qin Guo Department of Biology, Shandong University, 250100, Jinan, Shandong, P.R. China aut NATIONALLICENCE 700 1- Hui-min Chen Department of Biology, Shandong University, 250100, Jinan, Shandong, P.R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 155-158 0721-7714 11:3<155 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer