caa a22 4500 469027932 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232165 doi (NATIONALLICENCE)springer-10.1007/BF00232165 Transformation of opium poppy ( Papaver somniferum L.) with Agrobacterium rhizogenes MAFF 03-01724 [Elektronische Daten] [Kayo Yoshimatsu, Koichiro Shimomura] Summary: Transformed cultures of opium poppy (Papaver somniferum L.) were established by infecting hypocotyl segments with Agrobacterium rhizogenes MAFF 03-01724. Undifferentiated calli formed on the infected site grew satisfactorily on phytohormone-free solid medium in the dark and produced opine, mikimopine, which could not be detected in a normal culture. Numerous adventitious shoots developed from transformed calli during subculture. The transformed shoots separated individually were cultured on phytohormone-free MS solid medium at 22 ° C under 14 h/day light. They displayed wider leaves and longer internodes than shoots established from seeds or non-transformed root culture. The content of morphinan alkaloids in the cultures and regenerated shoots were quantitatively analyzed by enzyme-linked immunosorbent assay and high performance liquid chromatography. HPLC analysis revealed that non-transformed shoots contained much more codeine (1310 gmg/g dry wt.) than morphine (50 μg/g dry wt.), while the transformed shoot cultures did not contain morphine, although the level of morphinan alkaloids in the transformed shoots (213 μg morphine equivalents/g fr. wt.) was comparable to that in non-transformed shoots (182 μg morphine equivalents/g fr. wt.) by ELISA. Springer-Verlag, 1992 Transformation nationallicence Palaver somniferum nationallicence Agrobacterium rhizogenes nationallicence Morphinan alkaloids nationallicence ELISA nationallicence HPLC nationallicence MS : Murashige-Skoog (Murashige and Skoog 1962) nationallicence 1/2 MS : half strength MS nationallicence HF : phytohormone-free nationallicence NAA : 1-naphthaleneacetic acid nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence HPLC : high performance liquid chromatography nationallicence Yoshimatsu Kayo Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut Shimomura Koichiro Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 132-136 0721-7714 11:3<132 1992 11 299 https://doi.org/10.1007/BF00232165 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232165 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshimatsu Kayo Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Shimomura Koichiro Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 132-136 0721-7714 11:3<132 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer