caa a22 4500 469027940 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232162 doi (NATIONALLICENCE)springer-10.1007/BF00232162 Improved method for the transformation of Arabidopsis thaliana with chimeric dihydrofolate reductase constructs which confer methotrexate resistance [Elektronische Daten] [Elke Kemper, Christoph Grevelding, Jeff Schell, Robert Masterson] Summary: A modified root explant transformation method has been developed that is effective in producing transgenic Arabidopsis thaliana plants which are methotrexate resistant due to the integration of T-DNA vectors containing a chimeric dihydrofolate reductase gene. Molecular analysis shows that transformed methotrexate resistant plants contain the expected T-DNA construct with the chimeric gene. This transformation method also works well with other plant selectable markers, including hygromycin phosphotransferase and neomycin phosphotransferase II. Springer-Verlag, 1992 Kemper Elke Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany aut Grevelding Christoph Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany aut Schell Jeff Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany aut Masterson Robert Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 118-121 0721-7714 11:3<118 1992 11 299 https://doi.org/10.1007/BF00232162 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232162 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kemper Elke Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany aut NATIONALLICENCE 700 1- Grevelding Christoph Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany aut NATIONALLICENCE 700 1- Schell Jeff Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany aut NATIONALLICENCE 700 1- Masterson Robert Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, W-5000, Köln 30, Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 118-121 0721-7714 11:3<118 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer