caa a22 4500 469027959 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232167 doi (NATIONALLICENCE)springer-10.1007/BF00232167 Restoration of regeneration potential of long-term cultures of red fescue ( Festuca rubra L.) by elevated sucrose levels [Elektronische Daten] [O. Zaghmout, W. Torello] A tissue culture protocol for restoring embryogenic ability and increasing green plant regeneration from long-term callus (5-year old) and suspension cultures of "Dawson” red fescue (Festuca rubra var trichyoplylla Gaud) was developed. Pretreatment with elevated levels of sucrose over the standard level (60 mM) enhanced regeneration capacity and decreased the number of albino plants. The highest degree of embryogenesis and green shoot number occurred when calli were pre-treated on MS basal medium supplemented with 120 mM sucrose. Mannitol caused callus discoloration and death if added to pre-treatment media at 60, 90, 120, 150 or 180 mM. Cell suspension growth was greatest when 135 mM sucrose was added to the pre-treatment growth media. High concentrations of sucrose (135 and 180 mM) were necessary for plant regeneration from suspension aggregates pretreated with 135 or 180 mM sucrose and then plated on a growth regulator-free regeneration medium composed of half-strength MS salts and B5 vitamins. Springer-Verlag, 1992 Somatic embryogenesis nationallicence red fescue nationallicence sucrose nationallicence Zaghmout O. Department of Agronomy, Texas Tech University, 79409, Lubbock, TX, USA aut Torello W. Department of Plant and Soil Sciences, University of Massachusetts, 20 Stockbridge Hall, 01002, Amherst, MA, USA aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 142-145 0721-7714 11:3<142 1992 11 299 https://doi.org/10.1007/BF00232167 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232167 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zaghmout O. Department of Agronomy, Texas Tech University, 79409, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Torello W. Department of Plant and Soil Sciences, University of Massachusetts, 20 Stockbridge Hall, 01002, Amherst, MA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 142-145 0721-7714 11:3<142 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer