caa a22 4500 469027975 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232161 doi (NATIONALLICENCE)springer-10.1007/BF00232161 Genetic transformation of Medicago truncatula using Agrobacterium with genetically modified Ri and disarmed Ti plasmids [Elektronische Daten] [M. Thomas, R. Rose, K. Nolan] Summary: Fertile transgenic plants of the annual pasture legume Medicago truncatula were obtained by Agrobacterium-mediated transformation, utilising a disarmed Ti plasmid and a binary vector containing the kanamycin resistance gene under the control of the cauliflower mosaic virus 35S promoter. Factors contributing to the result included an improved plant regeneration protocol and the use of explants from a plant identified as possessing high regeneration capability from tissue culture. Genes present on the T-DNA of the Ri plasmid had a negative effect on somatic embryogenesis. Only tissue inoculated with Agrobacterium strains containing a disarmed Ti plasmid lacking the T-DNA region or a Ri plasmid with an inactivated rol A gene regenerated transgenic plants. Fertile transgenic plants were only obtained with disarmed A. tumefaciens, and the introduced NPT II gene was transmitted to R1 progeny. Springer-Verlag, 1992 Thomas M. Department of Biological Sciences, The University of Newcastle, 2308, New South Wales, Australia aut Rose R. Department of Biological Sciences, The University of Newcastle, 2308, New South Wales, Australia aut Nolan K. Department of Biological Sciences, The University of Newcastle, 2308, New South Wales, Australia aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 113-117 0721-7714 11:3<113 1992 11 299 https://doi.org/10.1007/BF00232161 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232161 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thomas M. Department of Biological Sciences, The University of Newcastle, 2308, New South Wales, Australia aut NATIONALLICENCE 700 1- Rose R. Department of Biological Sciences, The University of Newcastle, 2308, New South Wales, Australia aut NATIONALLICENCE 700 1- Nolan K. Department of Biological Sciences, The University of Newcastle, 2308, New South Wales, Australia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 113-117 0721-7714 11:3<113 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer