caa a22 4500 469027983 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232166 doi (NATIONALLICENCE)springer-10.1007/BF00232166 Transgenic poplars: expression of chimeric genes using four different constructs [Elektronische Daten] [Jean Leple, Ana Brasileiro, Marie Michel, Francis Delmotte, Lise Jouanin] Summary: Leaf or stem explants of a hybrid poplar clone (Populus tremula X Populus alba), sensitive to Agrobacterium tumefaciens, were co-cultivated either by an octopine or a nopaline disarmed A. tumefaciens modified strain. Transformed poplar shoots were readily regenerated from explants. The protocol was improved using the nopaline disarmed strain C58/pMP90 with the binary vector pBI121. This protocol was then used to test three other vectors. The first one, possessing a nptII gene fused to the CaMV 19S promoter, permitted regeneration of transformed shoots in presence of 50 to 100 mg/l kanamycin. The two other vectors carried an additional nptII gene under the control of the CaMV 35S or CaMV 35S promoter with a double enhancer sequence (CaMV 70). CaMV 70 promoter provided consistently higher level of gene expression than the other promoters in both callus and leaf tissues. Springer-Verlag, 1992 Leple Jean Station d'Amélioration des Arbres Forestiers, Institut National de la Recherche Agronomique, F-45160, Ardon, Olivet, France aut Brasileiro Ana Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026, Versailles Cedex, France aut Michel Marie aut Delmotte Francis Centre de Biophysique Moléculaire, Département de Biochimie des Glycoconjugués et Lectines Endogènes, Centre National de la Recherche Scientifique et Université d'Orléans, F-45071, Orléans Cedex 2, France aut Jouanin Lise Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026, Versailles Cedex, France aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 137-141 0721-7714 11:3<137 1992 11 299 https://doi.org/10.1007/BF00232166 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232166 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leple Jean Station d'Amélioration des Arbres Forestiers, Institut National de la Recherche Agronomique, F-45160, Ardon, Olivet, France aut NATIONALLICENCE 700 1- Brasileiro Ana Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Michel Marie aut NATIONALLICENCE 700 1- Delmotte Francis Centre de Biophysique Moléculaire, Département de Biochimie des Glycoconjugués et Lectines Endogènes, Centre National de la Recherche Scientifique et Université d'Orléans, F-45071, Orléans Cedex 2, France aut NATIONALLICENCE 700 1- Jouanin Lise Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026, Versailles Cedex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 137-141 0721-7714 11:3<137 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer