caa a22 4500 469027991 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232168 doi (NATIONALLICENCE)springer-10.1007/BF00232168 A non-invasive method for the routine-estimation of fresh weight of cells grown in batch suspension cultures [Elektronische Daten] [T. Blom, W. Kreis, F. van Iren, K. Libbenga] A simple apparatus was designed to allow sedimentation of plant cells grown in batch suspensions in Erlenmeyer flasks. After sedimentation the height of the cell mass along the glass wall was measured with a ruler fixed in the apparatus. The cell volume after sedimentation, calculated from this height, appeared highly correlated with the fresh weight of cells. This result was found with eight cell lines in two Laboratories. The method proved to be very suitable to allow routinely measurement of FW without the destruction of cells, from many samples, in a short time, during each phase of the growth cycle. Springer-Verlag, 1992 Blom T. Biotechnology Delft Leiden, Project-Group Plant Cell Biotechnology, Department of Plant Molecular Biology, Leiden University, Nonnensteeg 3, NL 2311, VJ Leiden, The Netherlands aut Kreis W. Pharmazeutisches Institut, Eberhard-Karls-Universität, Auf der Morgenstelle 8, W-7400, Tübingen, FRG aut van Iren F. Biotechnology Delft Leiden, Project-Group Plant Cell Biotechnology, Department of Plant Molecular Biology, Leiden University, Nonnensteeg 3, NL 2311, VJ Leiden, The Netherlands aut Libbenga K. Biotechnology Delft Leiden, Project-Group Plant Cell Biotechnology, Department of Plant Molecular Biology, Leiden University, Nonnensteeg 3, NL 2311, VJ Leiden, The Netherlands aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 146-149 0721-7714 11:3<146 1992 11 299 https://doi.org/10.1007/BF00232168 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232168 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blom T. Biotechnology Delft Leiden, Project-Group Plant Cell Biotechnology, Department of Plant Molecular Biology, Leiden University, Nonnensteeg 3, NL 2311, VJ Leiden, The Netherlands aut NATIONALLICENCE 700 1- Kreis W. Pharmazeutisches Institut, Eberhard-Karls-Universität, Auf der Morgenstelle 8, W-7400, Tübingen, FRG aut NATIONALLICENCE 700 1- van Iren F. Biotechnology Delft Leiden, Project-Group Plant Cell Biotechnology, Department of Plant Molecular Biology, Leiden University, Nonnensteeg 3, NL 2311, VJ Leiden, The Netherlands aut NATIONALLICENCE 700 1- Libbenga K. Biotechnology Delft Leiden, Project-Group Plant Cell Biotechnology, Department of Plant Molecular Biology, Leiden University, Nonnensteeg 3, NL 2311, VJ Leiden, The Netherlands aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 146-149 0721-7714 11:3<146 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer