caa a22 4500 469028009 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232172 doi (NATIONALLICENCE)springer-10.1007/BF00232172 Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius A., P. aureus (L.) Wilczek, P. coccineus L., and P. wrightii L [Elektronische Daten] [Kamal Malik, Praveen Saxena] A rapid, one-step procedure has been developed for inducing direct organogenesis and somatic embryogenesis in cultures of Phaseolus coccineus L., P. acutifolius A., P. aureus L. [Vigna radiata L. Wilczek] and P. wrightii L. Development of somatic embryos and shoot buds occurred within 6-8 weeks of culture from intact seedlings raised on MS (Murashige and Skoog 1962) medium supplemented with N6-benzylaminopurine (BAP). Shoot buds or embryoids originated from subepidermal tissue of the regions adjacent to the shoot apex, hypocotyl and cotyledonary axils. While P. acutifolius and P. aureus were regenerated via shoot formation and P. wrightii by somatic embryogenesis, both embryogenesis and shoot regeneration were observed in P. coccineus. Relatively higher levels of BAP, 50-80 μM, were found to be optimal for inducing regeneration while lower concentrations were ineffective. About 40-70 shoots and 70-250 somatic embryos were produced per responding seedling. Regenerated shoots and somatic embryos developed into whole plants on a basal medium or the one supplemented with 1 μM naphthaleneacetic acid. Springer-Verlag, 1992 N6-benzylaminopurine nationallicence Grain legumes nationallicence Organogenesis nationallicence Phaseolus nationallicence Somatic embryogenesis nationallicence Malik Kamal Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ont., Canada aut Saxena Praveen Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ont., Canada aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 163-168 0721-7714 11:3<163 1992 11 299 https://doi.org/10.1007/BF00232172 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232172 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malik Kamal Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ont., Canada aut NATIONALLICENCE 700 1- Saxena Praveen Department of Horticultural Science, University of Guelph, N1G 2W1, Guelph, Ont., Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 163-168 0721-7714 11:3<163 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer