caa a22 4500 469028025 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00232163 doi (NATIONALLICENCE)springer-10.1007/BF00232163 Repetitive somatic embryogenesis from peanut cultures in liquid medium [Elektronische Daten] [Richard Durham, Wayne Parrott] Summary: A regeneration system based on repetitive somatic embryogenesis was developed for peanut (Arachis hypogaea L.). Embryogenic suspension cultures were initiated using individual somatic embryos induced from immature cotyledons cultured on a modified Murashige and Skoog medium containing 40 mg/l 2,4-D for 30 days. After transfer to a modified MS liquid medium, the somatic embryos produced masses of secondary and tertiary embryos which continued to proliferate following manual separation and subculture of the embryogenic clumps. The cultures exhibited exponential growth, and have been maintained for over one year without apparent loss of embryogenic potential. Further embryo development, germination, and conversion were achieved by placing embryo clumps onto hormone-free, solid medium. The inclusion of a desiccation period during embryo development enhanced conversion four-fold. Plants have been established in soil and appear to be phenotypically normal. Springer-Verlag, 1992 Tissue culture nationallicence Regeneration nationallicence Arachis hypogaea nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence NAA : 1-naphthaleneacetic acid nationallicence BA : 6-benzylaminopurine nationallicence MSO : Modified Murashige and Skoog basal medium nationallicence EM : embryogenic masses nationallicence Durham Richard Department of Agronomy, The University of Georgia, 30602, Athens, GA, USA aut Parrott Wayne Department of Agronomy, The University of Georgia, 30602, Athens, GA, USA aut Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 122-125 0721-7714 11:3<122 1992 11 299 https://doi.org/10.1007/BF00232163 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232163 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Durham Richard Department of Agronomy, The University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Parrott Wayne Department of Agronomy, The University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/3(1992-04-01), 122-125 0721-7714 11:3<122 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer