caa a22 4500 469028041 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00236263 doi (NATIONALLICENCE)springer-10.1007/BF00236263 Improved plant regeneration from wheat anther and barley microspore culture using phenylacetic acid (PAA) [Elektronische Daten] [A. Ziauddin, A. Marsolais, E. Simion, K. Kasha] Summary: The effect of the auxin phenylacetic acid (PAA) on wheat anther and on barley anther/microspore culture was investigated. With PAA the induction response was not usually significantly different from controls but a significantly higher number of green plants were produced in wheat anther and barley microspore culture. For wheat anther culture 100 mg/L PAA was beneficial. For barley microspore culture the optimum levels were from 1 to 100 mg/L, depending on genotype. In barley anther culture there were no improvements using PAA. In wheat anther culture, 145 green plants/100 anthers were obtained with cultivar Veery‘S', while the average response from twelve F1 hybrids in the breeding program was 332 green plants/100 anthers. At least 1000 green plants were obtained using isolated microspores from 100 anthers in barley cv. Igri. With cv. Bruce, regeneration occurred only when 100 mg/L PAA was used. The influence of PAA appears at the embryogenic phase of the culture system. The possible mechanisms by which PAA may improve regeneration are discussed. Springer-Verlag, 1992 Ziauddin A. Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Marsolais A. Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Simion E. Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Kasha K. Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 489-498 0721-7714 11:10<489 1992 11 299 https://doi.org/10.1007/BF00236263 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236263 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ziauddin A. Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Marsolais A. Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Simion E. Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Kasha K. Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 489-498 0721-7714 11:10<489 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer