caa a22 4500 46902805X CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00236264 doi (NATIONALLICENCE)springer-10.1007/BF00236264 Stably transformed herbicide resistant callus of sugarcane via microprojectile bombardment of cell suspension cultures and electroporation of protoplasts [Elektronische Daten] [M. Chowdhury, Indra Vasil] Stably transformed callus of a hybrid sugarcane cultivar (Saccharum species hybrid, CP72-1210) was achieved following high velocity microprojectile bombardment of suspension culture cells, and electroporation of protoplasts. A three-day old cell suspension culture (SC88) was bombarded with gold particles coated with pBARGUS plasmid DNA containing the ß-glucuronidase (GUS) reporter gene and the bar selectable gene that confers resistance to the herbicide basta. The pBARGUS plasmid was also electroporated into the protoplasts of another cell line (SCPP). Colonies resistant to basta were recovered from both sources. Stable integration of the bar gene in the resistant cell lines was confirmed by Southern analysis. In addition, phosphinothricin acetyltransf erase (PAT) activity was also demonstrated in the transformed cell lines. Springer-Verlag, 1992 Sugarcane nationallicence cell suspension nationallicence protoplast nationallicence microprojectile bombardment nationallicence electroporation nationallicence GUS nationallicence bar nationallicence PAT nationallicence transformation nationallicence GUS : ß-glucuronidase nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence BAP : benzylaminopurine nationallicence PMSF : phenylmethylsulfonyl fluoride nationallicence MES : 2[N-Morpholino]ethanesulfonic acid nationallicence HEPES : [N-2-hydroxyethyl] piperazine-N′-[2-ethane sulfonic acid] nationallicence PAT : Phosphinothricin acetyltransferase nationallicence CTAB : cetyltrimethylammonium bromide nationallicence Chowdhury M. Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Florida, 32611-0660, Gainesville, FL, USA aut Vasil Indra Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Florida, 32611-0660, Gainesville, FL, USA aut Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 494-498 0721-7714 11:10<494 1992 11 299 https://doi.org/10.1007/BF00236264 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236264 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chowdhury M. Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Florida, 32611-0660, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Vasil Indra Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Florida, 32611-0660, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 494-498 0721-7714 11:10<494 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer