caa a22 4500 469028068 CHVBK 20180323132735.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00236267 doi (NATIONALLICENCE)springer-10.1007/BF00236267 Regeneration of shoots from embryo hypocotyls of common ash ( Fraxinus excelsior ) [Elektronische Daten] [A. Tabrett, N. Hammatt] Summary: The addition of thidiazuron (TDZ) to MS salts and vitamins, instead of benzylaminopurine (BAP) increased both the culture weight and the proportion of ash embryo hypocotyl explants that produced adventitious shoots. The concentration of BAP, but not that of TDZ also affected these parameters. Addition of 1-naphthalene acetic acid reduced culture performance, indolebutyric acid (IBA) was beneficial, and 2,4-dichlorophenoxyacetic acid had no effect. During both 1990 and 1991, rates of regeneration declined as the growth season progressed. Adventitious shoots, which were also obtained from hypocotyls from dried seeds, were established as proliferating shoot cultures following transfer to DKW medium with 5.0 mg l−1 BAP, and the resulting shoots were rooted in half-strength Woody Plant medium with 1.0 mg l−1 IBA. Springer-Verlag, 1992 Tabrett A. Horticulture Research International, East Malling, West Malling, ME19 6BJ, Kent, UK aut Hammatt N. Horticulture Research International, East Malling, West Malling, ME19 6BJ, Kent, UK aut Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 514-518 0721-7714 11:10<514 1992 11 299 https://doi.org/10.1007/BF00236267 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236267 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tabrett A. Horticulture Research International, East Malling, West Malling, ME19 6BJ, Kent, UK aut NATIONALLICENCE 700 1- Hammatt N. Horticulture Research International, East Malling, West Malling, ME19 6BJ, Kent, UK aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 514-518 0721-7714 11:10<514 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer