caa a22 4500 469028084 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00236271 doi (NATIONALLICENCE)springer-10.1007/BF00236271 Formation of multiple embryo-like structures from single microspores during maize anther culture [Elektronische Daten] [Y. Wan, J. Widholm] In a previous study on the RFLP analysis of the maize anther culture response (Wan et al. 1992), some of the anther-derived callus Unes from hybrids H99 x Pa91 (HP) and H99 x FR16 (HF) showed the same RFLP patterns with 58 (for HP Unes) or 35 (for HF lines) RFLP markers used. Since the callus Unes with the identical RFLP pattern were initiated from individual embryo-like structures (ELSs) from the same anther culture plate, these must have originated from the same microspore. Twin embryos which apparently had originated from the same microspore were also observed. Thus in certain cases one microspore must be capable of forming more than one ELS. However, in the case of the callus Unes from a different F1 hybrid (Pa91 x FR16), no identical RFLP patterns were observed. Thus multiple ELS formation from a single microspore may be genetically controlled. Since in some cases the proportion of callus lines resulting from multiple ELS formation can be quite high (about 50% for the HP lines), estimates of gene segregation and anther culture response frequencies can be affected greatly. Springer-Verlag, 1992 Zea mays L nationallicence Callus line nationallicence RFLP pattern nationallicence anther culture nationallicence Wan Y. Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 W. Gregory Ave., 61801, Urbana, IL, USA aut Widholm J. Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 W. Gregory Ave., 61801, Urbana, IL, USA aut Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 529-531 0721-7714 11:10<529 1992 11 299 https://doi.org/10.1007/BF00236271 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236271 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wan Y. Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 W. Gregory Ave., 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Widholm J. Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 W. Gregory Ave., 61801, Urbana, IL, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 529-531 0721-7714 11:10<529 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer