caa a22 4500 469028092 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00236268 doi (NATIONALLICENCE)springer-10.1007/BF00236268 Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum [Elektronische Daten] [P. Angeloni, H. Rey, L. Mroginski] Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and BAP, respectively. Shoots formed roots upon transfer to MS with 0.01 mg/l NAA. Plantlets were succesfully transferred to soil. Leaf-derived calli of Centrosema arenarium, C. macrocarpum, C. pascuorum, C. pubescens, and C. virginianum did not produce shoots when cultured in vitro. Springer-Verlag, 1992 Angeloni P. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Casilla de Correos 209, 3400, Corrientes, Argentina aut Rey H. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Casilla de Correos 209, 3400, Corrientes, Argentina aut Mroginski L. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Casilla de Correos 209, 3400, Corrientes, Argentina aut Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 519-521 0721-7714 11:10<519 1992 11 299 https://doi.org/10.1007/BF00236268 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236268 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Angeloni P. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Casilla de Correos 209, 3400, Corrientes, Argentina aut NATIONALLICENCE 700 1- Rey H. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Casilla de Correos 209, 3400, Corrientes, Argentina aut NATIONALLICENCE 700 1- Mroginski L. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Casilla de Correos 209, 3400, Corrientes, Argentina aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 519-521 0721-7714 11:10<519 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer