caa a22 4500 469028122 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00236265 doi (NATIONALLICENCE)springer-10.1007/BF00236265 Transformation and regeneration of Brassica rapa using Agrobacterium tumefaciens [Elektronische Daten] [Sharon Radke, Joann Turner, Daniel Facciotti] Summary: Transformation and regeneration procedures for obtaining transgenic Brassica rapa ssp. oleifera plants are described. Regeneration frequencies were increasedby using silver nitrate and by adjusting the duration of exposure to 2,4-D. For transformation, Agrobacterium tumefaciens strain EHA101 containing a binary plasmid with the neomycin phosphotransferase gene (NPT II) and the b-glucuronidase gene (GUS) was cocultivated with hypocotyl explants from the oilseed B. rapa cvs. Tobin and Emma. Transformed plants were obtained within three months of cocultivation. Transformation frequencies for the cultivars Tobin and Emma were 1-9%. Evidence for transformation was shown by NPT II dot blot assay, the GUS fluorometric assay, Southern analysis, and segregation of the kanamycin-resistance trait in the progeny. The transformation and regeneration procedure described here has been used routinely to transform two cultivars of B. rapa and 18 cultivars of B. napus. Springer-Verlag, 1992 Brassica nationallicence Oilseed rape nationallicence Gene transfer nationallicence Transgenic plants nationallicence Silver nitrate nationallicence Radke Sharon Calgene, Inc., 1920 Fifth Street, 95616, Davis, California, USA aut Turner Joann Calgene, Inc., 1920 Fifth Street, 95616, Davis, California, USA aut Facciotti Daniel Calgene, Inc., 1920 Fifth Street, 95616, Davis, California, USA aut Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 499-505 0721-7714 11:10<499 1992 11 299 https://doi.org/10.1007/BF00236265 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236265 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Radke Sharon Calgene, Inc., 1920 Fifth Street, 95616, Davis, California, USA aut NATIONALLICENCE 700 1- Turner Joann Calgene, Inc., 1920 Fifth Street, 95616, Davis, California, USA aut NATIONALLICENCE 700 1- Facciotti Daniel Calgene, Inc., 1920 Fifth Street, 95616, Davis, California, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 499-505 0721-7714 11:10<499 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer