caa a22 4500 469028130 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00236270 doi (NATIONALLICENCE)springer-10.1007/BF00236270 Plant regeneration from callus cultures of Piper longum L. by organogenesis [Elektronische Daten] [S. Bhat, A. Kackar, K. Chandel] Plant regeneration from callus cultures of Piper longum was achieved through organogenesis. In vitro grown shoots were used as explants for callus induction. Competent callus was initiated around the nodal ring of tissue using Murashige and Skoog medium supplemented with 1.0 mg.l−1α- naphthaleneacetic acid and 0.2 mg.l−1 N6-benzyladenine. Optimum growth regulator concentrations for shoot induction and shoot elongation were found to be 0.5 mg.l−1 indole-3-acetic acid with 1.5 mg.l−1 benzyladenine, and 0.1 mg.l−1 indole-3-acetic acid with 0.2 mg.l−1 benzyladenine, respectively. Elongated shoots were rooted on half-strength Murashige and Skoog medium having 0.1 mg.l−1 indole3-acetic acid. The rooted plants were successfully established in soil. Springer-Verlag, 1992 Bhat S. National Plant Tissue Culture Repository, NBPGR, Pusa Campus, 110012, New Delhi, India aut Kackar A. National Plant Tissue Culture Repository, NBPGR, Pusa Campus, 110012, New Delhi, India aut Chandel K. National Plant Tissue Culture Repository, NBPGR, Pusa Campus, 110012, New Delhi, India aut Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 525-528 0721-7714 11:10<525 1992 11 299 https://doi.org/10.1007/BF00236270 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00236270 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bhat S. National Plant Tissue Culture Repository, NBPGR, Pusa Campus, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Kackar A. National Plant Tissue Culture Repository, NBPGR, Pusa Campus, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Chandel K. National Plant Tissue Culture Repository, NBPGR, Pusa Campus, 110012, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/10(1992-09-01), 525-528 0721-7714 11:10<525 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer