caa a22 4500 469028165 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235086 doi (NATIONALLICENCE)springer-10.1007/BF00235086 Somatic embryogenesis and plant regeneration from zygotic embryo culture in blue spruce ( Picea pungens Engelman.) [Elektronische Daten] [John Afele, Tissa Senaratna, Bryan McKersie, Praveen Saxena] Summary: Somatic embryogenesis and plantlet formation have been achieved from cultured mature zygotic embryos of blue spruce (Picea pungens Engelman.). The effect of three basal media LP, LM, and BLG, all used at half-strength, was tested at the induction phase. LM medium induced somatic embryogenesis to a higher extent than LP whereas BLG did not produce any embryonal-suspensor mass representing stage 1 somatic embryos. The embryonal-suspensor mass was induced on a wide range of auxin/cytokinin ratios. However, media containing either 2 μM NAA and 10 μM BA, or 10 μM NAA and 5 μM BA produced somatic embryos that gave the highest frequency of plantlets. The level of ABA required in the maturation medium for somatic embryos to mature properly varied with the auxin/cytokinin levels in the induction medium on which the somatic embryos were derived. Inclusion of AgNO3 (10 - 100 μM) in the induction medium reduced somatic embryogenesis and embryo conversion. Springer-Verlag, 1992 Blue spruce nationallicence Somatic embryogenesis nationallicence Auxin and cytokinin ratio nationallicence Abscisic acid nationallicence NAA : naphthalene-acetic acid nationallicence BA : N6-benzylaminopurine nationallicence ABA : abscisic acid nationallicence Afele John Horticultural Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Senaratna Tissa Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut McKersie Bryan Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Saxena Praveen Horticultural Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 299-303 0721-7714 11:5-6<299 1992 11 299 https://doi.org/10.1007/BF00235086 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235086 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Afele John Horticultural Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Senaratna Tissa Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- McKersie Bryan Crop Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Saxena Praveen Horticultural Science Department, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 299-303 0721-7714 11:5-6<299 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer