caa a22 4500 469028181 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235070 doi (NATIONALLICENCE)springer-10.1007/BF00235070 Protoplast culture and plant regeneration of several species in the genus Dianthus [Elektronische Daten] [Masaru Nakano, Masahiro Mii] Summary: Seventeen cultivars belonging to the genus Dianthus were examined for protoplast isolation, culture and shoot regeneration under the same conditions. These included D. caryophyllus, D. chinensis, D. barbatus, D. plumarius, D. superbus and D. japonicus as well as interspecific hybrid cultivars (D. caryophyllus x D. chinensis and D. chinensis x D. barbatus). In all cultivars, viable protoplasts were isolated at high yields from leaves of axenic shoot cultures and some of these protoplasts divided and formed colonies. However, shoot regeneration frequencies were markedly different among the species. High frequency shoot regeneration was obtained from D. chinensis and interspecific hybrid cultivars, while only low frequency or no shoot regeneration was obtained from other species. Springer-Verlag, 1992 Nakano Masaru Plant Breeding Laboratory, Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut Mii Masahiro Plant Breeding Laboratory, Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 225-228 0721-7714 11:5-6<225 1992 11 299 https://doi.org/10.1007/BF00235070 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235070 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakano Masaru Plant Breeding Laboratory, Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut NATIONALLICENCE 700 1- Mii Masahiro Plant Breeding Laboratory, Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 225-228 0721-7714 11:5-6<225 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer