caa a22 4500 469028203 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235083 doi (NATIONALLICENCE)springer-10.1007/BF00235083 Regeneration of fertile plants from protoplasts of soybean (Glycine max L. Merr.): genotypic differences in culture response [Elektronische Daten] [Sarwan Dhir, Seema Dhir, Jack Widholm] Fourteen soybean (Glycine max L.) genotypes were evaluated for their regenerability from protoplasts using a procedure previously descibed for the cultivar Clark 63. Protoplasts were isolated from immature cotyledon tissue and were cultured in liquid or agarose gelled KP8, MS or B5 medium with different sugars. Significant differences were observed in plating efficiency, which was as high as 63% in Jack and A-2396, and as low as 38% in X-3337. Upon regular dilution with K8 medium, 1-2 mm diameter colonies were formed in 5-6 weeks with all the genotypes tested. These colonies were then transferred onto MSB (MS salts; Murashige & Skoog, 1962 + B5 organics; Gamborg et al., 1968) medium with 0.5 mg l−1 each of 2,4-D, BA and KN and 500 mg l−1 CH for further growth. Once the colonies had become green, compact and nodular, and were 8-10 mm in size, they were transferred to regeneration medium. Upon regular subculturing, calli of six genotypes; A-2396, Chamberlain, Heilong-26, Jack, Resnick and XP-3015 developed shoots, with the regeneration frequency being highest 27% in Jack (52 calli out of 192 produced 8-12 shoots). The regenerated shoots from different genotypes were elongated and rooted. So far, sixty three complete plants have been obtained, including twelve A-2396, nineteen Chamberlain, fifteen Jack, nine Resnick and eight XP-3015. A total of thirty five plants have been transplanted into pots in the greenhouse. Sixteen have set seeds and others are producing flowers and pods. Springer-Verlag, 1992 Dhir Sarwan Plant and Animal Biotechnology Laboratory, Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 West Gregory, 61801, Urbana, IL, USA aut Dhir Seema Plant and Animal Biotechnology Laboratory, Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 West Gregory, 61801, Urbana, IL, USA aut Widholm Jack Plant and Animal Biotechnology Laboratory, Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 West Gregory, 61801, Urbana, IL, USA aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 285-289 0721-7714 11:5-6<285 1992 11 299 https://doi.org/10.1007/BF00235083 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235083 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dhir Sarwan Plant and Animal Biotechnology Laboratory, Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 West Gregory, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Dhir Seema Plant and Animal Biotechnology Laboratory, Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 West Gregory, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Widholm Jack Plant and Animal Biotechnology Laboratory, Department of Agronomy, University of Illinois at Urbana-Champaign, 1201 West Gregory, 61801, Urbana, IL, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 285-289 0721-7714 11:5-6<285 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer