caa a22 4500 469028211 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235082 doi (NATIONALLICENCE)springer-10.1007/BF00235082 Contrasting pattern of somatic and zygotic embryo development in alfalfa ( Medicago sativa L.) as revealed by scanning electron microscopy [Elektronische Daten] [Nanfei Xu, J. Bewley] Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos developing in ovulo. Somatic embryos begin as distinct pro-embryos within the callus tissue pieces placed in suspension culture. They become globular and heart-shaped while on solid agar medium and then undergo cotyledon elongation and maturation. Somatic embryos develop comparatively slower at early stages of development and faster at the later stages than zygotic embryos. They lack a well-defined suspensor and have a very rough, poorly-differentiated epidermis, the first layer of which is lost after pro-embryo formation. The cotyledons of somatic embryos are multiple and poorlydeveloped; there appears to be a correlation between the amount of surface roughness of the developing embryo and the extent to which polycotyledony occurs. Springer-Verlag, 1992 Medicago sativa nationallicence embryogenesis nationallicence somatic embryo nationallicence scanning electron microscopy nationallicence Xu Nanfei Department of Botany, University of Guelph, N1G 2W1, Ontario, Guelph, Canada aut Bewley J. Department of Botany, University of Guelph, N1G 2W1, Ontario, Guelph, Canada aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 279-284 0721-7714 11:5-6<279 1992 11 299 https://doi.org/10.1007/BF00235082 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235082 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Nanfei Department of Botany, University of Guelph, N1G 2W1, Ontario, Guelph, Canada aut NATIONALLICENCE 700 1- Bewley J. Department of Botany, University of Guelph, N1G 2W1, Ontario, Guelph, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 279-284 0721-7714 11:5-6<279 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer