caa a22 4500 469028246 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235090 doi (NATIONALLICENCE)springer-10.1007/BF00235090 Somatic hybridization between Brassica juncea and Moricandia arvensis by protoplast fusion [Elektronische Daten] [P. Kirti, S. Narasimhulu, Shyam Prakash, V. Chopra] Intergeneric somatic hybrids have been produced between Brassica juncea (2n=36, AABB) cv. RLM-198 and Moricandia arvensis (2n=28, MM) by protoplast fusion. Hypocotyl protoplasts of B. juncea were fused with mesophyll protoplasts of M. arvensis using polyethylene glycol. Fusion frequency, estimated on the basis of differential morphological characterstics of parental protoplasts was about 5%. Of the 156 calli obtained, four calli produced shoots intermediate in morphology between the parents. Hybrid nature of the plants was confirmed using wheat nuclear rDNA probe. Hybridization of total DNA with a mitochondrial DNA probe carrying 5s-18s rRNA genes of maize showed that the mitochondria of the somatic hybrids were derived from the wild species M. arvensis. Meiosis in the only hybrid that produced normal flowers revealed the occurrence of 64 chromosomes, the sum of chromosomes of parental species. Inspite of complete pollen sterility, siliquas were produced in this hybrid by back-crossing with B. juncea. These siliquas on in vitro culture produced 12 seeds. Springer-Verlag, 1992 Brassica juncea nationallicence Moricandia arvensis nationallicence Protoplast fusion nationallicence Intergeneric somatic hybrid nationallicence Kirti P. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Narasimhulu S. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Prakash Shyam Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Chopra V. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 318-321 0721-7714 11:5-6<318 1992 11 299 https://doi.org/10.1007/BF00235090 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235090 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kirti P. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Narasimhulu S. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Prakash Shyam Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Chopra V. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 318-321 0721-7714 11:5-6<318 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer