caa a22 4500 469028254 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235078 doi (NATIONALLICENCE)springer-10.1007/BF00235078 Plant regeneration from long term suspension culture-derived protoplasts of hexaploid wheat ( Triticum aestivum L.) [Elektronische Daten] [Yao-Min Qiao, Marzia Cattaneo, Franca Locatelli, Elisabetta Lupotto] Highly regenerable callus cultures have been obtained from immature embryos of hexaploid wheat cv. Oderzo. Friable fast growing calli were induced at high frequency. Suspensions were initiated from the most friable callus lines: they became established in about two months. Suspensions consisted of cell aggregates of 30 to 1000 um in diameter. Upon plating on MS hormone-free medium, suspensions regenerated green plantlets, and their regenerative capability was maintained for at least 10 months. Protoplasts were isolated from 7-8 day old suspension cultures with a yield of 4-6×106 protoplasts/g fresh weight cells. Protoplast culture was either in liquid medium or in a bead-type system with agarose beads. First divisions were detected at day 5. At day 14 visible colonies were detected and the plating efficiency was evaluated between 2 and 8% over the initial number of protoplasts plated. Protoplast-derived calli were cultured in the presence of 1 mg/l IAA and 0.5 mg/l zeatin and were used for reinitiating new suspension cultures. Upon plating onto MS hormone-free medium, with or without the addition of 0.1 mg/l GA3, calliclones were induced to differentiate. Regeneration of complete plantlets, with shoot and roots took about two months. Plantlets were grown in sterile conditions until 12-15 cm height, and were subsequently transplanted in soil. Springer-Verlag, 1992 Triticum aestivum nationallicence embryogenic suspension cultures nationallicence protoplasts nationallicence regeneration nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence IAA : indole-3-acetic acid nationallicence MS : Murashige and Skoog (1962) medium nationallicence PCV : packed cell volume nationallicence MES : morpholinoethanesulfonic acid nationallicence Qiao Yao-Min Istituto Sperimentale per la Cerealicoltura, Via Mulino 3, 20079, S. Angelo, Lodigiano, MI, Italy aut Cattaneo Marzia Istituto Sperimentale per la Cerealicoltura, Via Mulino 3, 20079, S. Angelo, Lodigiano, MI, Italy aut Locatelli Franca Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy aut Lupotto Elisabetta Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 262-265 0721-7714 11:5-6<262 1992 11 299 https://doi.org/10.1007/BF00235078 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235078 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qiao Yao-Min Istituto Sperimentale per la Cerealicoltura, Via Mulino 3, 20079, S. Angelo, Lodigiano, MI, Italy aut NATIONALLICENCE 700 1- Cattaneo Marzia Istituto Sperimentale per la Cerealicoltura, Via Mulino 3, 20079, S. Angelo, Lodigiano, MI, Italy aut NATIONALLICENCE 700 1- Locatelli Franca Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy aut NATIONALLICENCE 700 1- Lupotto Elisabetta Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100, Bergamo, Italy aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 262-265 0721-7714 11:5-6<262 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer