caa a22 4500 469028262 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235077 doi (NATIONALLICENCE)springer-10.1007/BF00235077 Somatic embryogenesis and plant regeneration from zygotic embryo explants in mexican weeping bamboo, Otatea acuminata aztecorum [Elektronische Daten] [Susan Woods, Gregory Phillips, John Woods, Glenn Collins] An efficient protocol has been developed for the in vitro propagation of Mexican Weeping Bamboo through somatic embryogenesis from zygotic embryo explants. Mature seeds and excised embryos were cultured in the light or in the dark on both Murashige and Skoog's and Gamborg's B5 basal media with various supplements. Optimal somatic embryogenesis and plant regeneration were obtained by culture in the dark on Murashige and Skoog's basal medium supplemented with 3 mg/1 2,4-dichlorophenoxyacetic acid, 0.5 mg/1 6-benzylaminopurine and 2.0% sucrose. More than 95% of the germinating somatic embryos developed shoots and roots, and were transferred to soil with 85% success. Springer-Verlag, 1992 Woods Susan Department of Agronomy, University of Kentucky, 40546-0091, Lexington, KY, USA aut Phillips Gregory Department of Agronomy & Horticulture, New Mexico State Univ., 88003-0003, Las Craces, NM, USA aut Woods John Department of Agronomy, University of Kentucky, 40546-0091, Lexington, KY, USA aut Collins Glenn Department of Agronomy, University of Kentucky, 40546-0091, Lexington, KY, USA aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 257-261 0721-7714 11:5-6<257 1992 11 299 https://doi.org/10.1007/BF00235077 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235077 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Woods Susan Department of Agronomy, University of Kentucky, 40546-0091, Lexington, KY, USA aut NATIONALLICENCE 700 1- Phillips Gregory Department of Agronomy & Horticulture, New Mexico State Univ., 88003-0003, Las Craces, NM, USA aut NATIONALLICENCE 700 1- Woods John Department of Agronomy, University of Kentucky, 40546-0091, Lexington, KY, USA aut NATIONALLICENCE 700 1- Collins Glenn Department of Agronomy, University of Kentucky, 40546-0091, Lexington, KY, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 257-261 0721-7714 11:5-6<257 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer