caa a22 4500 469028289 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235069 doi (NATIONALLICENCE)springer-10.1007/BF00235069 Transgenic herbicide-resistant Atropa belladonna using an Ri binary vector and inheritance of the transgenic trait [Elektronische Daten] [Kazuki Saito, Mami Yamazaki, Hiroyuki Anzai, Katsuyoshi Yoneyama, Isamu Murakoshi] Summary: Transgenic Atropa belladonna conferred with a herbicide-resistant trait was obtained by transformation with an Ri plasmid binary vector and plant regeneration from hairy roots. We made a chimeric construct, pARK5, containing the bar gene encoding phosphinothricin acetyltransferase flanked with the promoter for cauliflower mosaic virus 35S RNA and the 3′ end of the nos gene. Leaf discs of A. belladonna were infected with Agrobacterium rhizogenes harboring an Ri plasmid, pRi15834, and pARK5. Transformed hairy roots resistant to bialaphos (5 mg/l) were selected and plantlets were regenerated. The integration of T-DNAs from pRi15834 and pARK5 were confirmed by DNA-blot hybridization. Expression of the bar gene in transformed R0 tissues and in backcrossed F1 progeny with a nontransformant and self-fertilized progeny was indicated by enzymatic activity of the acetyltransferase. The transgenic plants showed resistance towards bialaphos and phosphinothricin. Tropane alkaloids of normal amounts were produced in the transformed regenerants. These results present a successful application of transformation with an Ri plasmid binary vector for conferring an agronomically useful trait to medicinal plants. Springer-Verlag, 1992 Saito Kazuki Faculty of Pharmaceutical Sciences, Chiba University, Yayoicho 1-33, 260, Chiba, Japan aut Yamazaki Mami Faculty of Pharmaceutical Sciences, Chiba University, Yayoicho 1-33, 260, Chiba, Japan aut Anzai Hiroyuki Pharmaceutical Research Center, Meiji Seika Kaisha Ltd., Kohokuku, 222, Yokohama, Japan aut Yoneyama Katsuyoshi Faculty of Agriculture, Meiji University, Tamaku, 214, Kawasaki, Japan aut Murakoshi Isamu Faculty of Pharmaceutical Sciences, Chiba University, Yayoicho 1-33, 260, Chiba, Japan aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 219-224 0721-7714 11:5-6<219 1992 11 299 https://doi.org/10.1007/BF00235069 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235069 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito Kazuki Faculty of Pharmaceutical Sciences, Chiba University, Yayoicho 1-33, 260, Chiba, Japan aut NATIONALLICENCE 700 1- Yamazaki Mami Faculty of Pharmaceutical Sciences, Chiba University, Yayoicho 1-33, 260, Chiba, Japan aut NATIONALLICENCE 700 1- Anzai Hiroyuki Pharmaceutical Research Center, Meiji Seika Kaisha Ltd., Kohokuku, 222, Yokohama, Japan aut NATIONALLICENCE 700 1- Yoneyama Katsuyoshi Faculty of Agriculture, Meiji University, Tamaku, 214, Kawasaki, Japan aut NATIONALLICENCE 700 1- Murakoshi Isamu Faculty of Pharmaceutical Sciences, Chiba University, Yayoicho 1-33, 260, Chiba, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 219-224 0721-7714 11:5-6<219 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer