caa a22 4500 469028297 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235071 doi (NATIONALLICENCE)springer-10.1007/BF00235071 Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 ( Oryza sativa L.) [Elektronische Daten] [Karabi Datta, Ingo Potrykus, Swapan Datta] Protoplasts were isolated from embryogenic cell suspensions obtained from mature seed derived embryogenic callus of the advanced Indica type rice breeding line IR72 available from IRRI (International Rice Research Institute), Manila. Culture of protoplasts with the agarose bead type method without nurse culture led to sustained proliferation of protoplast derived clones. A simple culture protocol was developed which stimulated embryogenic development. Germination of somatic embryos has so far produced 277 green plants from 6 independent experiments. 117 plants have been transferred to soil and are growing in the greenhouse. A few of them have already flowered and set seeds. Springer-Verlag, 1992 Datta Karabi ETH-Zurich, Institute of Plant Sciences, CH-8092, Zurich, Switzerland aut Potrykus Ingo ETH-Zurich, Institute of Plant Sciences, CH-8092, Zurich, Switzerland aut Datta Swapan ETH-Zurich, Institute of Plant Sciences, CH-8092, Zurich, Switzerland aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 229-233 0721-7714 11:5-6<229 1992 11 299 https://doi.org/10.1007/BF00235071 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235071 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Datta Karabi ETH-Zurich, Institute of Plant Sciences, CH-8092, Zurich, Switzerland aut NATIONALLICENCE 700 1- Potrykus Ingo ETH-Zurich, Institute of Plant Sciences, CH-8092, Zurich, Switzerland aut NATIONALLICENCE 700 1- Datta Swapan ETH-Zurich, Institute of Plant Sciences, CH-8092, Zurich, Switzerland aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 229-233 0721-7714 11:5-6<229 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer