caa a22 4500 469028300 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235074 doi (NATIONALLICENCE)springer-10.1007/BF00235074 Agrobacterium mediated transfer of a mutant Arabidopsis acetolactate synthase gene confers resistance to chlorsulfuron in chicory ( Cichorium intybus L.) [Elektronische Daten] [A. Vermeulen, H. Vaucheret, V. Pautot, Y. Chupeau] Summary: Leaf discs of C. intybus were inoculated with an Agrobacterium tumefaciens strain harboring a neomycin phosphotransferase (neo) gene for kanamycin resistance and a mutant acetolactate synthase gene (csr1-1) from Arabidopsis thaliana conferring resistance to sulfonylurea herbicides. A regeneration medium was optimized which permitted an efficient shoot regeneration from leaf discs. Transgenic shoots were selected on rooting medium containing 100 mg/l kanamycin sulfate. Integration of the csr1-1 gene into genomic DNA of kanamycin resistant chicory plants was confirmed by Southern blot hybridizations. Analysis of the selfed progenies (S1 and S2) of two independent transformed clones showed that kanamycin and chlorsulfuron resistances were inherited as dominant Mendelian traits. The method described here for producing transformed plants will allow new opportunities for chicory breeding. Springer-Verlag, 1992 Cichorium intybus nationallicence Agrobacterium tumefaciens nationallicence Chlorsulfuron resistance nationallicence Kanamycin resistance nationallicence Vermeulen A. Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut Vaucheret H. Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut Pautot V. Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut Chupeau Y. Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 243-247 0721-7714 11:5-6<243 1992 11 299 https://doi.org/10.1007/BF00235074 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235074 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vermeulen A. Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Vaucheret H. Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Pautot V. Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Chupeau Y. Laboratoire de Biologie Cellulaire, INRA, F-78026, Versailles Cedex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 243-247 0721-7714 11:5-6<243 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer