caa a22 4500 469028319 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235081 doi (NATIONALLICENCE)springer-10.1007/BF00235081 Agrobacterium tumefaciens -mediated beta-glucuronidase (GUS) gene expression in lentil ( Lens culinaris Medik.) tissues [Elektronische Daten] [Thomas Warkentin, Alan McHughen] Summary: Lentil (Lens culinaris Medik.) shoot apex, epicotyl, and root expiants were capable of expressing an intron-containing beta-glucuronidase (GUS) gene after inoculation with the disarmed Agrobacterium strain GV2260:p35SGUSINT. Expression occurred at all wound sites on these expiants except at the end of the root expiants proximal to the cotyledonary node. GUS expression was detected using both histochemical and fluorescence assays and was stable for at least nine days after inoculation for epicotyl and root expiants, and for at least seventeen days for shoot apices. Non-inoculated controls, or controls inoculated with an Agrobacterium strain lacking the GUS gene, did not produce any background blue staining in the histochemical assay. Expression levels for all lentil expiants were substantially lower than for comparable flax (Linum usitatissimum L.) expiants which served as a positive control. Springer-Verlag, 1992 Beta-glucuronidase nationallicence Lentil nationallicence Lens culinaris Medik nationallicence Transformation nationallicence Agrobacterium tumefaciens nationallicence Warkentin Thomas Crop Development Centre, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut McHughen Alan Crop Development Centre, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 274-278 0721-7714 11:5-6<274 1992 11 299 https://doi.org/10.1007/BF00235081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Warkentin Thomas Crop Development Centre, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- McHughen Alan Crop Development Centre, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 274-278 0721-7714 11:5-6<274 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer