caa a22 4500 469028343 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235088 doi (NATIONALLICENCE)springer-10.1007/BF00235088 Visualization of vacuoplasts in isolated vacuole preparations from mesophyll protoplasts of periwinkle [ Catharanthus roseus (L.) G. Don] [Elektronische Daten] [David McCaskill, A. Scott] A procedure was developed for the rapid detection of vacuoplasts in vacuole preparations isolated from mesophyll protoplasts of Catharanthus roseus (L.) G. Don (periwinkle). The procedure relies on the staining of surface carbohydrates on the plasma membrane surrounding vacuoplasts with fluorescein-labeled lectins. When isolated under conditions of constant osmotic strength, approximately 15-20% of the vacuoles isolated showed surface labeling with FITC-agglutinin from Abrus precatorius. Isolation of vacuoles after an initial osmotic shock showed much lower (<5%) surface labeling. This lower level of surface labeling correlated well with a lower level of other non-vacuolar marker enzyme activities. A thin layer of cytoplasm was visible in a small number of these stained structures, indicating that they were vacuoplasts. Springer-Verlag, 1992 Catharanthus nationallicence Lectin nationallicence Protoplast nationallicence Vacuole nationallicence FITC : fluorescein isothiocyanate nationallicence McCaskill David Department of Biochemistry and Biophysics, Texas A&M University, 77843, College Station, Texas, USA aut Scott A. Department of Biochemistry and Biophysics, Texas A&M University, 77843, College Station, Texas, USA aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 310-313 0721-7714 11:5-6<310 1992 11 299 https://doi.org/10.1007/BF00235088 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235088 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McCaskill David Department of Biochemistry and Biophysics, Texas A&M University, 77843, College Station, Texas, USA aut NATIONALLICENCE 700 1- Scott A. Department of Biochemistry and Biophysics, Texas A&M University, 77843, College Station, Texas, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 310-313 0721-7714 11:5-6<310 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer