caa a22 4500 469028351 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235085 doi (NATIONALLICENCE)springer-10.1007/BF00235085 Recovery of plants from cryopreserved embryogenic cell suspensions of Pinus caribaea [Elektronische Daten] [Eric Laine, Pascale Bade, Alain David] Summary: Embryogenic cell suspension cultures of Pinus caribaea var. hondurensis have been cryopreserved in liquid nitrogen for up to four months, using sucrose and dimethylsulfoxide as cryoprotectants. Post-thaw growth was obtained after a short lag phase. Removal of the remaining liquid around the cells using a filter disc favoured subsequent regrowth of the cells. These reestablished cultures maintained an embryogenic potential similar to non-frozen cultures. The embryos produced were able to regenerate into plants, which are now growing in a greenhouse. Springer-Verlag, 1992 Cryopreservation nationallicence Pinus caribaea nationallicence embryogenic cell suspension nationallicence BA : 6-benzyladenine nationallicence DMSO : dimethylsulfoxide nationallicence FDA : fluorescein diacetate nationallicence MS : Murashige and Skoog nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence PAR : photosynthetically active radiation nationallicence Laine Eric Laboratoire de Biotechnologies Végétales, Université de Picardie, Faculté des Sciences, 33, rue Saint-Leu, 80039, Amiens Cedex, France aut Bade Pascale Laboratoire de Biotechnologies Végétales, Université de Picardie, Faculté des Sciences, 33, rue Saint-Leu, 80039, Amiens Cedex, France aut David Alain Laboratoire de Biotechnologies Végétales, Université de Picardie, Faculté des Sciences, 33, rue Saint-Leu, 80039, Amiens Cedex, France aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 295-298 0721-7714 11:5-6<295 1992 11 299 https://doi.org/10.1007/BF00235085 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235085 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Laine Eric Laboratoire de Biotechnologies Végétales, Université de Picardie, Faculté des Sciences, 33, rue Saint-Leu, 80039, Amiens Cedex, France aut NATIONALLICENCE 700 1- Bade Pascale Laboratoire de Biotechnologies Végétales, Université de Picardie, Faculté des Sciences, 33, rue Saint-Leu, 80039, Amiens Cedex, France aut NATIONALLICENCE 700 1- David Alain Laboratoire de Biotechnologies Végétales, Université de Picardie, Faculté des Sciences, 33, rue Saint-Leu, 80039, Amiens Cedex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 295-298 0721-7714 11:5-6<295 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer