caa a22 4500 469028378 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00235072 doi (NATIONALLICENCE)springer-10.1007/BF00235072 In vitro culture of isolated microspores and regeneration of plants in Brassica campestris [Elektronische Daten] [A. Baillie, D. Epp, D. Hutcheson, W. Keller] A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microspores were isolated from buds 2.0 - 2.9 mm in length and cultured in modified Lichter (1982) medium containing 17% sucrose, pH 6.2. After 48 h at 32°C, the incubation medium was replaced with NLN (Lichter 1982) medium containing 10% sucrose. Microspores were cultured at 24°C in darkness and embryos developed after three weeks. More than 1000 plants have thus far been regenerated. Genotypic differences were observed for microspore embryogenesis. The majority of the regenerants were haploid, however colchicine could be effectively used to achieve chromosome doubling. Springer-Verlag, 1992 Brassica campestris nationallicence Microspore nationallicence Embryogenesis nationallicence Haploid nationallicence Baillie A. Plant Biotechnology Institute, 110 Gymnasium Road, S7N OW9, Saskatoon, Canada aut Epp D. Plant Biotechnology Institute, 110 Gymnasium Road, S7N OW9, Saskatoon, Canada aut Hutcheson D. Agriculture Canada Research Station, 107 Science Crescent, S7N OX2, Saskatoon, Canada aut Keller W. Plant Biotechnology Institute, 110 Gymnasium Road, S7N OW9, Saskatoon, Canada aut Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 234-237 0721-7714 11:5-6<234 1992 11 299 https://doi.org/10.1007/BF00235072 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235072 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baillie A. Plant Biotechnology Institute, 110 Gymnasium Road, S7N OW9, Saskatoon, Canada aut NATIONALLICENCE 700 1- Epp D. Plant Biotechnology Institute, 110 Gymnasium Road, S7N OW9, Saskatoon, Canada aut NATIONALLICENCE 700 1- Hutcheson D. Agriculture Canada Research Station, 107 Science Crescent, S7N OX2, Saskatoon, Canada aut NATIONALLICENCE 700 1- Keller W. Plant Biotechnology Institute, 110 Gymnasium Road, S7N OW9, Saskatoon, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/5-6(1992-06-01), 234-237 0721-7714 11:5-6<234 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer