caa a22 4500 469028408 CHVBK 20180323132736.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00232531 doi (NATIONALLICENCE)springer-10.1007/BF00232531 Transformation of vitis tissue by different strains of Agrobacterium tumefaciens containing the T-6b gene [Elektronische Daten] [R. Berres, L. Otten, B. Tinland, E. Malgarini-Clog, B. Walter] Summary: Stem pieces and leaf disks of Vitis spp. were cocultured with Agrobacterium tumefaciens strains carrying the UidA (ß-glucuronidase = GUS) gene. The transformation efficiency was highly increased by using a modified T-6b gene (a gene from pTiTm4) which interferes with normal growth and allows regeneration of normal Nicotiana rustica plants (Tinland 1990). The strains first tested on stem segments were subsequently tested in a leaf explant system. On leaves the transformation efficiency of the strains was much lower than with stems. Both the T-6b gene and the hsp 70-T-6b gene (a modified T-6b gene under the control of a heat shock promoter) allowed the initiation of GUS-positive buds. Springer-Verlag, 1992 Grapevine nationallicence Agrobacterium tumefaciens nationallicence transformation nationallicence GUS : ß-glucuronidase nationallicence BAP : benzylaminopurine nationallicence X-gluc : 5-bromo-4-chloro-3-indolyl glucuronide nationallicence Berres R. Institut National de Recherche Agronomique, Station Vigne et Vin, Laboratoire de Pathologie Végétale, 28, rue de Herrlisheim, 68021, Colmar Cedex, France aut Otten L. Institut de Biologie Moléculaire des Plantes, Laboratoire de Phytopathologie Moléculaire, 12, rue du Général Zimmer, 67084, Strasbourg Cedex, France aut Tinland B. Institut de Biologie Moléculaire des Plantes, Laboratoire de Phytopathologie Moléculaire, 12, rue du Général Zimmer, 67084, Strasbourg Cedex, France aut Malgarini-Clog E. Institut National de Recherche Agronomique, Station Vigne et Vin, Laboratoire de Pathologie Végétale, 28, rue de Herrlisheim, 68021, Colmar Cedex, France aut Walter B. Institut National de Recherche Agronomique, Station Vigne et Vin, Laboratoire de Pathologie Végétale, 28, rue de Herrlisheim, 68021, Colmar Cedex, France aut Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 192-195 0721-7714 11:4<192 1992 11 299 https://doi.org/10.1007/BF00232531 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232531 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berres R. Institut National de Recherche Agronomique, Station Vigne et Vin, Laboratoire de Pathologie Végétale, 28, rue de Herrlisheim, 68021, Colmar Cedex, France aut NATIONALLICENCE 700 1- Otten L. Institut de Biologie Moléculaire des Plantes, Laboratoire de Phytopathologie Moléculaire, 12, rue du Général Zimmer, 67084, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Tinland B. Institut de Biologie Moléculaire des Plantes, Laboratoire de Phytopathologie Moléculaire, 12, rue du Général Zimmer, 67084, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Malgarini-Clog E. Institut National de Recherche Agronomique, Station Vigne et Vin, Laboratoire de Pathologie Végétale, 28, rue de Herrlisheim, 68021, Colmar Cedex, France aut NATIONALLICENCE 700 1- Walter B. Institut National de Recherche Agronomique, Station Vigne et Vin, Laboratoire de Pathologie Végétale, 28, rue de Herrlisheim, 68021, Colmar Cedex, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 192-195 0721-7714 11:4<192 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer