caa a22 4500 469028416 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00232532 doi (NATIONALLICENCE)springer-10.1007/BF00232532 Factors influencing the efficiency of T-DNA transfer during co-cultivation of Antirrhinum majus with Agrobacterium tumefaciens [Elektronische Daten] [P. Holford, N. Hernandez, H. Newbury] Summary: The effects of varying the pH of the cocultivation medium, additons of vir-inducing phenolic compounds and the strains of wild-type agrobacteria on transformation rates of a number of different varieties of Antirrhinum majus were studied. In general, optimal transformation was found with strains C58 or A281 and was favoured by low pH and the inclusion of acetosyringone in the co-cultivation medium. However, maximal transformation of the least susceptible variety was achieved at high pH and in the presence of syringaldehyde. This demonstrates the need for the optimization of a wide range of culture conditions when working with new genotypes and offers a rational approach towards the development of Agrobacterium-mediated transformation of new species or varieties. Springer-Verlag, 1992 Holford P. School of Biological Sciences, University of Birmingham, B15 2TT, Birmingham, UK aut Hernandez N. School of Biological Sciences, University of Birmingham, B15 2TT, Birmingham, UK aut Newbury H. School of Biological Sciences, University of Birmingham, B15 2TT, Birmingham, UK aut Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 196-199 0721-7714 11:4<196 1992 11 299 https://doi.org/10.1007/BF00232532 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232532 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holford P. School of Biological Sciences, University of Birmingham, B15 2TT, Birmingham, UK aut NATIONALLICENCE 700 1- Hernandez N. School of Biological Sciences, University of Birmingham, B15 2TT, Birmingham, UK aut NATIONALLICENCE 700 1- Newbury H. School of Biological Sciences, University of Birmingham, B15 2TT, Birmingham, UK aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 196-199 0721-7714 11:4<196 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer