caa a22 4500 469028424 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00232529 doi (NATIONALLICENCE)springer-10.1007/BF00232529 Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter [Elektronische Daten] [Tadaaki Hashimoto, Tetsuji Yamada, Akiko Tada, Shinji Kawamata, Yoshikazu Tanaka, Pernpong Sriprasertsak, Yuki Ichinose, Hisaharu Kato, Satoshi Izutsu, Tomonori Shiraishi, Hachiro Oku, Yoshiaki Ohtsuki] Summary: High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 μg/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen. Springer-Verlag, 1992 Pisum sativum L. Midoriusui nationallicence Protoplast nationallicence Suspension culture nationallicence Phenylalanine ammonia-lyase nationallicence Transient assay nationallicence CAT : chloramphenicol acetyltransferase nationallicence PAL : phenylalanine ammonia-lyase nationallicence CM : acetylated chloramphenicol nationallicence GSH : reduced glutathione nationallicence NOS : nopaline synthase nationallicence ES : electroporation solution nationallicence CaMV : cauliflower mosaic virus nationallicence GUS : β-glucuronidase nationallicence CHS : chalcone synthase nationallicence 2,4-D : 2, 4-dichlorophenoxyacetic acid nationallicence Hashimoto Tadaaki Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut Yamada Tetsuji Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut Tada Akiko Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut Kawamata Shinji aut Tanaka Yoshikazu aut Sriprasertsak Pernpong aut Ichinose Yuki Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut Kato Hisaharu Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut Izutsu Satoshi Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut Shiraishi Tomonori Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut Oku Hachiro Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut Ohtsuki Yoshiaki Agricultural Research Center, Kannondai 3-1-1, 305, Tsukuba, Japan aut Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 183-187 0721-7714 11:4<183 1992 11 299 https://doi.org/10.1007/BF00232529 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232529 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hashimoto Tadaaki Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Yamada Tetsuji Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Tada Akiko Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Kawamata Shinji aut NATIONALLICENCE 700 1- Tanaka Yoshikazu aut NATIONALLICENCE 700 1- Sriprasertsak Pernpong aut NATIONALLICENCE 700 1- Ichinose Yuki Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Kato Hisaharu Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Izutsu Satoshi Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Shiraishi Tomonori Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Oku Hachiro Laboratory of Plant Pathology & Genetic Engineering, College of Agriculture, Okayama University, Tsushima, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Ohtsuki Yoshiaki Agricultural Research Center, Kannondai 3-1-1, 305, Tsukuba, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 183-187 0721-7714 11:4<183 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer