caa a22 4500 469028432 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00232528 doi (NATIONALLICENCE)springer-10.1007/BF00232528 Polymerase chain reaction comparison of the gene for strictosidine synthase from ten Rauvolfia species [Elektronische Daten] [Daniel Bracher, Toni Kutchan] The gene for strictosidine synthase, str1, has been analyzed by the polymerase chain reaction in ten species of Rauvolfia, the origins of which span the tropical belt: R. cambodiana (Indochina), R. canescens (India), R. chinensis (China), R. heterophylla (Central America), R. mannii (West Africa), R. nitida (West Indies), R. praecox (Brasil), R. serpentina (India), R. sumatrana (Indonesia) and R. verticillata (Indochina). Restriction endonuclease analysis of the gene fragments produced with genomic DNA from each of the ten species as template revealed that str1 is highly conserved in the Rauvolfia species investigated. These results suggest that there is a stringent selection pressure on the gene for this key enzyme of indole alkaloid biosynthesis. Springer-Verlag, 1992 Bracher Daniel Lehrstuhl für Pharmazeutische Biologie, Universität München, Karlstraße 29, 2, München, Germany aut Kutchan Toni Lehrstuhl für Pharmazeutische Biologie, Universität München, Karlstraße 29, 2, München, Germany aut Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 179-182 0721-7714 11:4<179 1992 11 299 https://doi.org/10.1007/BF00232528 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232528 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bracher Daniel Lehrstuhl für Pharmazeutische Biologie, Universität München, Karlstraße 29, 2, München, Germany aut NATIONALLICENCE 700 1- Kutchan Toni Lehrstuhl für Pharmazeutische Biologie, Universität München, Karlstraße 29, 2, München, Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 179-182 0721-7714 11:4<179 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer