caa a22 4500 469028440 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00232530 doi (NATIONALLICENCE)springer-10.1007/BF00232530 Expression of an abscisic acid responsive promoter in Picea abies (L.) Karst. following bombardment from an electric discharge particle accelerator [Elektronische Daten] [Ronald Newton, Haile Yibrah, Niu Dong, David Clapham, Sara von Arnold] The 1.5 kilobase promoter sequence upstream of Dc8, a late embryo abundant gene of Daucus, fused to the reporter β-glucuronidase gene was introduced into several tissues of Picea abies via a custom-made electric-discharge particle accelerator. Transient expression was measured histochemically as spot number 2 d after bombardment. Embryogenic suspensions gave higher levels of expression depending upon cell line than embryogenic callus or zygotic embryos. Expression was enhanced when cultures were treated with abscisic acid for 3 d before bombardment. A mean and maximum of 17 and 34 spots/disk, respectively, were observed with the best cell line, which was comparable with the level of expression driven by an enhanced 35S promoter. Springer-Verlag, 1992 Newton Ronald Department of Forest Science, Texas Agricultural Experiment Station, Texas A & M University System, 77843-2135, College Station, TX aut Yibrah Haile Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-75007, Uppsala, Sweden aut Dong Niu Department of Forest Science, Texas Agricultural Experiment Station, Texas A & M University System, 77843-2135, College Station, TX aut Clapham David Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-75007, Uppsala, Sweden aut von Arnold Sara Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-75007, Uppsala, Sweden aut Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 188-191 0721-7714 11:4<188 1992 11 299 https://doi.org/10.1007/BF00232530 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232530 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Newton Ronald Department of Forest Science, Texas Agricultural Experiment Station, Texas A & M University System, 77843-2135, College Station, TX aut NATIONALLICENCE 700 1- Yibrah Haile Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-75007, Uppsala, Sweden aut NATIONALLICENCE 700 1- Dong Niu Department of Forest Science, Texas Agricultural Experiment Station, Texas A & M University System, 77843-2135, College Station, TX aut NATIONALLICENCE 700 1- Clapham David Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-75007, Uppsala, Sweden aut NATIONALLICENCE 700 1- von Arnold Sara Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-75007, Uppsala, Sweden aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 188-191 0721-7714 11:4<188 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer