caa a22 4500 469028459 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00232526 doi (NATIONALLICENCE)springer-10.1007/BF00232526 Ultrastructural changes in suspension culture cells of Panicum maximum during cryopreservation [Elektronische Daten] [Shashi Gnanapragasam, Indra Vasil] A Panicum maximum cell suspension was used to study ultrastructural changes during cryopreservation. Pregrowing the cells in mannitol caused reduction in the vacuolar volume by redistribution of the large central vacuole into a number of smaller vesicles. Invaginations were formed in the plasma membrane of the cells, to accommodate the reduced cell volume. Swelling of organelles occurred during different stages of cryopreservation. The cisternae of the endoplasmic reticulum dilated and formed vesicles. Although some damage was apparent, organelles were still recognizable in cells frozen slowly and freeze-fixed at −10°C. The cells were able to repair such damage within two days in culture, and regained their normal appearance. Cells frozen slowly without any cryoprotection, and cells frozen rapidly by direct immersion into liquid nitrogen after cryoprotection, were lethally damaged by destruction of membranous structures. Osmiophilic granules were found along the plasma membrane of lethally damaged cells, indicating that their formation is a consequence of freeze damage, rather than a mechanism to prevent injury. Springer-Verlag, 1992 Cryopreservation nationallicence Panicum maximum nationallicence ultrastructure nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence DMSO : dimethyl sulfoxide nationallicence Gnanapragasam Shashi Laboratory of Plant Cell and Molecular Biology, Department of Vegetable Crops, University of Florida, 32611-0514, Gainesville, FL, USA aut Vasil Indra Laboratory of Plant Cell and Molecular Biology, Department of Vegetable Crops, University of Florida, 32611-0514, Gainesville, FL, USA aut Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 169-174 0721-7714 11:4<169 1992 11 299 https://doi.org/10.1007/BF00232526 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232526 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gnanapragasam Shashi Laboratory of Plant Cell and Molecular Biology, Department of Vegetable Crops, University of Florida, 32611-0514, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Vasil Indra Laboratory of Plant Cell and Molecular Biology, Department of Vegetable Crops, University of Florida, 32611-0514, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 169-174 0721-7714 11:4<169 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer